血管紧张素转换酶和醛固酮合成酶基因多态性与原发性高血压病的关系研究  

Associations of Polymorphisms of Angiotensin Converting Enzyme Gene and Aldosterone Synthase Gene with Essential Hypertension

作　　者：龚洪涛[1] 马先林[2] 杜凤和[1] 

机构地区：[1]首都医科大学附属北京天坛医院心内科,北京市100050 [2]北京健宫医院心内科

出　　处：《中国全科医学》2011年第23期2609-2611,共3页Chinese General Practice

摘　　要：目的探讨血管紧张素转换酶(ACE)基因的插入/缺失(I/D)多态性和醛固酮合成酶(CYP11B2)基因-344C/T多态性与原发性高血压病(EH)之间的关系。方法应用聚合酶链反应(PCR)对200例汉族EH患者和192例汉族正常血压者的ACE基因I/D多态性和CYP11B2基因-344C/T多态性进行检测,测定空腹血糖、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)等指标,分析各基因型和等位基因频率与EH的关系。结果 ACE I/D各基因型在EH组和正常对照组间差异有统计学意义(P=0.022),ACE D等位基因在EH组中的频率明显高于对照组(0.49和0.37,P=0.012)。用Logistic回归模型校正了传统危险因素的影响后,ACE DD基因型的携带者患高血压的危险性显著增加(P=0.003),OR值为3.66〔95%CI(1.54,8.69);〕EH组和对照组CYP11B2-344C/T各基因型与等位基因频率比较,差异均无统计学意义(P>0.05)。结论 ACE DD基因型与EH发病相关,可能是高血压发病的风险因子,CYP11B2基因-344T/C多态性不能独立预测高血压的患病风险。Objective To investigate the associations of the insertion/deletion（I/D） polymorphism of angiotensin converting enzyme（ACE） gene and the 344C/T polymorphism of Aldosterone synthase（CYP11B2） gene with essential hypertension（EH）.Methods The study covered 200 hypertension patients and 192 normotensive controls.The polymorphisms of ACE I/D and CYP11B2-344C/T were were determined by polymerase chain reaction（PCR）.Biochemical indices including serum glucose（GLU）,total cholesterol（TC）,triglyceride（TG）,high density lipoprotein（HDL） and low density lipoprotein（LDL）were also measured.Associations of genotype and allele frequencies with EH were analyzed.Results The polymorphism of ACE I/D between the EH group and the control group showed significant differences（P=0.022）;the frequency of ACE gene D allele in the EH group was notably higher than in the control group（0.49 vs 0.37,P=0.012）.ACE gene DD genotype was shown to be correlated with higher risk of EH after other risk factors being adjusted for by logistic regression analysis（P=0.003,OR=3.66,95%CI（1.54,8.69））.There was no significant difference in CYP11B2-344C/T genotype and allele frequencies between the case group and the control group.Conclusion ACE DD genetype is associated with essential hypertension and may be the risk factor of hypertension.The 344C/T polymorphism of CYP11B2 gene doesn′t qualify as an independent risk factor of EH.

关 键 词：血管紧张素转换酶 醛固酮合酶 基因多态性 原发性高血压 

分 类 号：R544.1[医药卫生—心血管疾病]