细胞外基质金属蛋白酶诱导因子、基质金属蛋白酶9及P38丝裂原活化蛋白激酶在子宫内膜异位症中的表达及相关性  

The expression and correlation of EMMPRIN,MMP-9 and P38 MAPK in endometriosis

作　　者：张婧[1] 唐良萏[1] 周敏[1] 龙行涛[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《中国实用妇科与产科杂志》2011年第8期595-598,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：国家自然科学基金资助(基金编号:30772034);重庆市科委计划项目(编号:2007BB5316)

摘　　要：目的探讨细胞外基质金属蛋白酶诱导因子(extracellular matrix metalloproteinase inducer,EMMPRIN)、基质金属蛋白酶9(matrix metalloproteinases-9,MMP-9)和P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)在子宫内膜异位症(endometriosis,EMs)中的表达及相关性。方法收集2008年5-12月重庆医科大学附属第一医院收治的EMs患者44例(异位内膜44例,在位内膜38例),对照组为同期非EMs患者在位内膜34例。应用免疫组化SABC三步法检测各组织中EMMPRIN、MMP-9、P38及磷酸化P385(p-P38)蛋白的表达,并对各蛋白表达水平进行相关性分析。结果 EMMPRIN、MMP-9、P38、p-P38蛋白在EMs异位内膜表达均高于EMs在位内膜组及对照组在位内膜,差异有统计学意义(P<0.05);Spearman相关分析显示,在EMs异位内膜中各蛋白表达呈正相关(P<0.05)。在EMs异位内膜组中各蛋白表达Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期,差异均有统计学意义(P<0.05)。EMs在位内膜组与对照组相比,各蛋白表达差异无统计学意义(P>0.05)。结论 EMMPRIN、MMP-9在EMs异位内膜中呈显著高表达并呈正相关性,在EMs的发生发展过程中可能起重要作用。EMMPRIN诱导刺激MMP-9高表达可能与p38 MAPK途径有关。Objective To investigate the expression and association of the extracellular matrix metalloproteinase inducer （ EMMPRIN）, the matrix metalloproteinases-9 （ MMP-9 ）, and the P38 mitogen-activated protein kinase （ MAPK ） in patients diagnosed with endometriosis（EMs）. Methods The expression of EMMPRIN,MMP-9, P38, p-P38 was detected by SABC immunohistochemistry in 44 patients with EMs （44 ectopic endometrium,38 eutopic endometrium） , and compared with 34 normal endometrium with non-endornetriosis. Correlation analysis was done. Results The positive expression rates of EMMPRIN, MMP-9, P38 and p-P38 in the ectopic endometrium with EMs were significantly higher than the eutopic endometrium group and the control group（ P 〈 0. 05 ）. Spearman correlation analysis showed that positive correlation was found in EMMPRIN, MMP-9, P38 and p-P38 in the eetopic endometrium group（P 〈0. 05）. The expression level of EMMPRIN ,MMP-9 ,P38 and p-P38 in the stages of III - IV displayed significantly higher than those in I - II in the ectop- ic endometrium group（P 〈0. 05）. The positive expression rates were no significant difference between the eutopic endometrium group and the control group（ P 〉 0. 05 ）. Conclusion The expression of EMMPRIN, MMP-9 in the ectopic endometrium with EMs was significantly higher and positive correlation, and they may play an important role in implantation and development. P38 MAPK may have a pathophysiological role in EMs.

关 键 词：关键词:子宫内膜异位症 细胞外基质金属蛋白酶诱导因子 基质金属蛋白酶9 P38丝裂原活化蛋白激酶 免疫组织化学 

分 类 号：R711.71[医药卫生—妇产科学]