小花棘豆化学成分预试及薄层色谱分析  

Chemical Test and TLC Analysis of Oxytropis glabra DC

作　　者：陈基萍[1] 牟新瑞 白松 赵宝玉[1] 王占新[4] 李蓉[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]乌鲁木齐正大畜牧有限公司,乌鲁木齐830088 [3]西藏农牧厅畜牧技术推广总站,拉萨850000 [4]中国农业大学动物医学院,北京100083

出　　处：《西北农业学报》2011年第6期66-70,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金项目(31072175)

摘　　要：为给小花棘豆化学成分研究及其各萃取部分指纹图谱的建立提供依据,采用植物化学成分系统预试法、薄层色谱分析技术及平面色谱图像定量法对小花棘豆化学成分进行分析。结果表明,小花棘豆含有油脂、酚类、鞣质、生物碱、黄酮及其苷类、皂甙、有机酸、蒽醌类等化合物,无强心苷、氰甙和脂肪族硝基化合物;石油醚、氯仿、乙酸乙酯和正丁醇萃取部分至少含有9、7、6和5种化合物,经与标准品对照,证明正丁醇萃取部分含有苦马豆素;各萃取部分相对含量最高的斑点依次为1、7、5、1,小花棘豆化学成分主要集中在石油醚和正丁醇萃取部分。For further investigating the chemical principals of Oxytropis glabra DC and providing theoretical bases to prevention and treatment of locoweed poisoning and developing the medicinal value.Phytochemistry composition preliminary systematic and special tests and thin layer chromatography（TLC） were applied to analyze the chemical principals.The results showed that the plant contained oils,phenols,tannin,alkaloids,flavonoids,terpenoids,Saponins organic acid,chinones,et al.but no cardiac glycoside,cyanophoric glycoside and aliphatic nitro compounds;10 kg materials were reflux extracted with industrial alcohol for four times and 1 167.5 g extractum was obtained.After dissolving with distilled water,the extractum was extracted with petroleum ether,chloroform,ethyl acetate,and n-butanol in turn.Four parts were separately obtained 172.23,12.64,30.71,180.5 g,which indicated that the principals were distributed in petroleum ether and n-butanol fractions;it was showed that 9,7,6 and 5 kinds of principals were located in the four fractions after analyzed by TLC and swainsonine was contained in n-butanol fraction when compared with the proof sample.The silica gel plates best developed were analyzed to determine the contents with planar chromatography image quantitative software,the results showed that Rf1,Rf7,Rf5,Rf1 points were the maximum content points in the four fractions respectively.

关 键 词：小花棘豆 化学成分 苦马豆素 薄层色谱 含量测定 

分 类 号：S452[农业科学—植物保护]