重组毕赤酵母体系生成PlUGT1蛋白适宜条件研究  

STUDIES ON APPRORIATE CONDITIONS FOR EXPRESSING PlUGT1 PROTEIN IN RECOMBINANT PICHIA PASTORIS SYSTEM

作　　者：胡小祥[1] 吕旻[1] 周文灵[1] 李玲[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2011年第3期103-106,共4页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省科技计划项目(2009B020301003)

摘　　要：通过单因素试验研究pH和温度对重组毕赤酵母GS115/pPIZαA-P lUGT1生长以及甲醇体积分数、pH和温度对诱导表达P lUGT1蛋白的影响.结果表明,pH6.5和30℃是培养重组毕赤酵母GS115/pPIZαA-P lUGT1最佳的生长条件,重组毕赤酵母GS115/pPIZαA-P lUGT1诱导表达蛋白的适宜条件为甲醇体积分数0.5%、30℃和pH5.0,该条件下培养72 h,培养液中的蛋白质量浓度为0.721 mg/mL.Based on the single factor experiment,the effects of pH values and temperatures on growth of GS115/pPIZαA-PlUGT1,and the effects of methanol concentrations,pH values and temperature on PlUGT1 protein production were studied.The optimal growth conditions of GS115/pPIZαA-PlUGT1 were as follows： pH 6.5 and 30℃.The optimal expression conditions of PlUGT1 protein were found to be 0.5% of methanol concentration,30℃ and pH 5.0.In this expression conditions for 72 h culture,the protein concentration in cultural solution is 0.721 mg/mL.

关 键 词：重组毕赤酵母 PlUGT1蛋白 生长条件 表达 

分 类 号：Q93[生物学—微生物学]