重组人γ-干扰素摇瓶生产工艺优化  

Optimization of Production Technology of Recombinant Human Interferon-γ in Shaking Flask

作　　者：许崇利[1] 胡静涛[2] 许崇波[3] 

机构地区：[1]吉林化工学院环境与生物工程学院,吉林132022 [2]吉林农业大学动物科学技术学院,长春130118 [3]大连大学医学院,辽宁大连116622

出　　处：《中国兽药杂志》2011年第8期15-19,共5页Chinese Journal of Veterinary Drug

基　　金：国家自然科学基金项目资助(30972188)

摘　　要：通过研究不同类型培养基和培养温度、起始pH值等发酵条件对rhIFN-γ产量的影响,初步确认了摇瓶水平发酵各因素的最优条件,优化的发酵条件为发酵起始pH值7.0、培养温度37℃、IPTG诱导浓度1.0 mmol/L、菌体生长密度OD600达到1.0时加入IPTG、诱导时间为3 h。此时rhIFN-γ产量达到最高水平,从而为rhIFN-γ批量生产奠定了良好的基础。The expression level of rhIFN - γ was influenced by the different medium and other fermentation parameters including culture temperature, initial pH and so on. The optimal parameters of various factors were confirmed by flask -fermentation. The optimal fermentation parameters with IPTG induction were： culture medium pH7.0, culture temperature 37 ℃, joining IPTG to final concentration 1.0 mmol/L when the recombinant strain growth density OD600 achieved 1. 0, and induction time 3 h. The production of rhIFN - γreached the highest level. This fermentation technology established a good foundation for the rhIFN -γmass production.

关 键 词：重组人Γ-干扰素 摇瓶发酵 表达 

分 类 号：R979.5[医药卫生—药品]