猪殃殃提取物不同极性部位抗白血病活性比较  

Comparison of Anti-leukemia Activity among Different Polar Parts of Galium aparine L. Extracts

作　　者：时国庆[1,2] 赵文恩[1] 王永胜[3] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001 [2]郑州轻工业学院食品与生物工程学院,河南郑州450002 [3]河南煤化集团新乡中新化工有限责任公司,河南新乡453800

出　　处：《安徽农业科学》2011年第20期12149-12150,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]比较猪殃殃提取物不同极性部位的抗白血病活性。[方法]采用95%乙醇对猪殃殃进行回流提取,得到浸膏再进行索氏极性梯度分级,经白血病K562细胞体外抗癌活性检验,以确定猪殃殃不同极性部位和生物活性的关系。[结果]猪殃殃各极性部位正丁醇部位得率最高,其次是二氯甲烷;浸膏和各极性部位对K562细胞的生长都具有一定的抑制作用,各部位抑制活性均优于浸膏。其中乙醚、二氯甲烷、乙酸乙酯部位抑制活性较强且相差不大,正丁醇部位抑制作用较弱。[结论]猪殃殃提取物含有潜在的抗癌活性成分,可用于开发新型抗肿瘤药物。[Objective] To compare effects of different polar parts of Galium aparine L.extracts on anti-leukemia activity.[Method] Galium aparine L.was refluxed with 95% ethanol and the obtained extracts were separated into different polar parts by Soxhlet extractor.The relationship between polar parts and biological activity was investigated through leukemia cells-K562 in vitro antitumor activity test.[Result] The content of extractum was the highest in butanol part,followed by dichloromethane part.The extractum and polar parts had a certain inhibitory activity on the growth of K562 cells,in which the latter was better than the former.Ether,dichloromethane and ethyl acetate polarity components had the stronger inhibitory activity,while normal butanol phase＇s inhibition was weak.[Conclusion] Extracts of Galium aparine L.contained some anti-cancer components that could be used in developing new anticancer drugs.

关 键 词：猪殃殃(Galium aparine L.) MTT 白血病 

分 类 号：S567[农业科学—中草药栽培]