miRNA-181b在牛腺垂体中的表达规律及其调控作用研究  

Study on the Expression Regulation and Regulatory Functions of miRNA-181b in the Anterior Pituitary of Cattle

作　　者：许橙[1] 薛文志[1] 赵茂鑫[1] 陈信[1] 刘颖[1] 于汪洋[1] 许艳丽[1] 马腾壑[1] 高妍[2] 张嘉保[2] 袁宝[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《安徽农业科学》2011年第20期12213-12215,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然基金项目(30972100);吉林省科技发展计划项目(201101015)

摘　　要：[目的]探索m iRNA-181b在成熟（18月龄）和未成熟（1月龄）牛腺垂体中差异表达量及其调控功能。[方法]通过构建成熟（18月龄）和未成熟（1月龄）的延边黄牛腺垂体组织m iRNA cDNA文库,对文库中的m iRNA进行Solexa高通量深度测序,并对公牛腺垂体中的m iRNA进行鉴别。从测序结果中挑选出具有差异表达的m iRNA-181b,经实时荧光定量PCR验证其在不同发育期延边黄牛腺垂体中的表达规律,并通过TargetScanS预测m iRNA-181b的靶基因。[结果]m iRNA-181b在不同发育期牛腺垂体表达量存在显著差异,1月龄公牛腺垂体中m iRNA-181b表达量是18月龄的4.05倍。m iRNA-181b与FSHβ基因3＇非编码区838~844碱基可能是特异结合,其结合碱基为UGAAUGUA。[结论]该试验为FSHβ的转录后调控研究提供了理论依据。[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary and its regulation function.[Method] cDNA library of miRNA in mature（18-month-old） and immature（one-month-old） cattle＇s anterior pituitary were established.After Solexa high-throughput sequencing of miRNA in the cDNA library,miRNA in anterior pituitary of bulls was identified.miRNA-181b with differential expression were selected from the sequencing results.By real-time quantitative RT-PCR,the expression regulation of miRNA-181b in the anterior pituitary of Yanbian Cattle in different growth period was validated.And the target genes of miRNA-181b were forecast by using TargetScanS.[Result] The expression quantity of miRNA-181b had great difference in cattle＇s anterior pituitary different growth periods.The expression quantity of miRNA-181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle.The binding of miRNA-181b with 838~844 bases in 3＇ untranslated region of FSHβ gene was specific and the binding base sites were UGAAUGUA.[Conclusion] This experiment provided the theoretical basis for the transcription regulation research of FSHβ.

关 键 词：MIRNA 实时荧光定量PCR 差异表达 

分 类 号：S823[农业科学—畜牧学]