微生物转化左旋四氢巴马汀产生左旋紫堇达明高产菌株的紫外诱变选育  

Screening of l-Corydalmine High-producing Strain Catalyzing the Biotransformation of l-Tetrahydropalmatine by UV Mutatigenesis

作　　者：徐田[1] 刘吉华[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药复方研究室,南京211198

出　　处：《药物生物技术》2011年第4期313-316,共4页Pharmaceutical Biotechnology

基　　金："十一五"重大新药创制项目(2009ZXJ09004-085)资助

摘　　要：紫堇达明为中药元胡中高效、微量活性成分,灰色链霉菌(Streptomyces griseus ATCC 13273)具有转化左旋四氢巴马汀产生左旋紫堇达明的生物转化活性。本研究采用紫外诱变法对原始菌株进行诱变处理,以期获得左旋紫堇达明高产突变菌株。正交试验结果显示:孢子浓度为107个/mL,照射距离为30 cm,照射时间为30 s为最佳诱变条件。筛选得到的突变株UV-056对底物的转化率达到62.58%,左旋紫堇达明转化产率达到33.12%,分别较出发菌株提高了51.00%和67.63%。检测表明,突变株经连续传代后仍可保持较好的遗传稳定性。l - corydalmine （l - CDL）, the active component in tratitionai Chinese medicillct plant Corydalis yanhusuo W. T. Wang, was a trace constitution with high efficiency. Streptomyces griseus ATCC 13273 catalyzed the microbial transformation process of l - tetrahydropalmatine （l - THP） to produce l - CDL. In this study, Streptomyces griseus ATCC 13273 was subjected to an ultraviolet radiation treatment in order to breed a l - CDL highproducing mutant strain. Orthogonal test suggested that the optimal condition of UV mutation was radiation for 30 s at a distance of 30 cm with a spore concentration adjusted to 107 units/ml. After screening process, a l - CDL high - producing mutant strain UV - 056 was obtained. The transformation rate of l- THP and the yield of l- CDL reached to 62. 58% and 33. 12% respectively, which was increased hy 51.00% and 67.63% compared with the original strain. The result indicated that the obtained mutant strain UV - 056 showed good genetic stability after continuous passage.

关 键 词：灰色链霉菌ATCC 13273 紫堇达明 紫外诱变 

分 类 号：Q812[生物学—生物工程]