不同粒径的固态及胶体纳米粒子体内动力学特性的比较  

Comparison of Dynamic Properties Between Different Size of Solid and Colloid Nanoparticles in vivo

作　　者：吴振华[1] 孙冰丽[2] 张建[1] 陈海燕[1] 顾月清[1] 

机构地区：[1]中国药科大学生命科学与技术学院生物医学工程研究室,南京210009 [2]江苏江山制药有限公司,江苏靖江214500

出　　处：《药物生物技术》2011年第4期344-347,376,共5页Pharmaceutical Biotechnology

摘　　要：纳米粒子在生物医学工程研究领域有着广阔的应用前景,其体内分布及清除等体内动力学特性对开发其体内应用有着极其重要的指导意义。本文分别对不同粒径的固态及胶体纳米粒子在小鼠动物模型体内的分布及清除等动态过程进行了详尽的研究。其中水凝胶纳米粒子由自由基沉淀聚合法合成,通过调控反应液中表面活性剂的浓度而获得不同粒径的纳米水凝胶,并将其包裹近红外荧光探针而进行体内动力学特性的在位研究;固态纳米粒子则通过合成Si O2包裹的近红外荧光量子点获得,通过控制Si O2外壳厚度而控制其粒径。近红外成像结果表明:不同粒径的胶体纳米粒子在体内的分布没有明显的组织特异性,但其清除时间随着粒径的增大而延长;而不同粒径的固态纳米粒子在体内的分布具有明显的特异性,为体内各种疾病的诊断提供了实时动态数据。Nanoparticles own highly extensive prospect in the fields of biomedical engineering research and play a significant role in the development of their application for human beings according to their dynamic characteristics in vivo, such as distribution and clearance. In this study, the dynamic processes of distribution and clearance using colloid and solid nanoparticle with different particle sizes in mouse model was investigated. The nano-hydrogel was synthesized using the method of precipitation polymerization and their different particle sizes were obtained by regulating the concentration of surfactant. To investigate their dynamic characteristics in vim, near-infrared fluorescent probe were encapsulated in the nano-hydrogel. The solid nanoparticles were prepared by SiO2-coated near-infrared fluorescent quantum dots and the diameters of diferent particles were obtained by changing the thickness of SiO2-shell. The results of near-infrared imaging indicated that there was no obvious tissue specificity about the nano-hydrogel with different particle sizes in vivo, while the clearance time prolonged with the increasing of the particle size. Different from nano-hydrogel, specific performance in distribution were obviously found out during the studies of solid nanoparticle with various particle sizes. Thus, it can provide real-time dynamic data when used for the diagnosis of various diseases.

关 键 词：纳米粒子 近红外 体内动力学 在位 靶向性 

分 类 号：Q7[生物学—分子生物学]