小刺猴头液体深层发酵浸膏多糖提取工艺的优化及分离纯化  

Optimization of Extraction Technology and Isolation Purification of Polysaccharides from Hericium caput-medusae Liquid Submerged Fermentation Extractum

作　　者：王新宇[1,2] 柳洪芳[1,3] 沈思捷[1,2] 韩铁军[4] 杨晓兵[1,2] 宋慧[1,2] 

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]吉林农业大学食药用菌教育部工程研究中心,长春130118 [3]黑龙江省农垦科学院测试化验中心,佳木斯154007 [4]白求恩医科大学制药厂,长春130012

出　　处：《吉林农业大学学报》2011年第5期536-540,544,共6页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20030551-1);国家科技支撑计划课题任务书项目(2007BAI38B06-03)

摘　　要：采用热水浸提法提取小刺猴头液体深层发酵浸膏多糖,在液料比、提取时间和提取温度单因素试验的基础上,利用SAS 9.2(the SAS System for Windows 9.2)软件设计响应面,以多糖得率作为响应值,优化得最佳提取条件:液料比(mL/g)2.29∶1,提取时间4.52 h,提取温度79.5℃,2次平行多糖得率12.28%。通过乙醇分级、脱蛋白、透析、过凝胶柱分离多糖,采用醋酸纤维素薄膜电泳、柱层析、紫外光谱及反复冻融鉴定为均一多糖。Warm water extraction was used for Hericium caput-medusae liquid submerged fermentation extractum polysaccharides,based on solvent-solid ratio,extracting time,extracting temperature single-factor experiments.The SAS 9.2 software was employed to design response surface and the yield of polysaccharides was used as the responsive values.The optimum ultrasonie-assisted extraction conditions were as follows： solvent/solid ratio 2.29∶1（mL/g）,extracting time 4.52 h,extracting temperature 79.5℃,the yield of polysaccharides rate was 12.28 %.Ethyl alcohol fractionation,purified through deproteinization,dialysis and freeze-dry.The polysaccharides were identified as uniform after film electrophoresis,column chromatography,the spectrum of ultraviolet spectra and repeat freeze thawing.

关 键 词：小刺猴头 液体深层发酵浸膏 多糖 热水浸提法 响应面法 醋酸纤维素薄膜电泳 

分 类 号：R284[医药卫生—中药学]