HPLC法测定麦冬中沿阶草酮甲的含量  

Determination of the Content of Ophiopogonanone A in Ophiopogonis Radix by HPLC

作　　者：侯浣波[1] 周慧[2,3] 余萍[4] 曾品涛[2,3] 郑一敏[2,3] 

机构地区：[1]四川省第五人民医院,四川成都610031 [2]重庆理工大学药学与生物工程学院,重庆400050 [3]重庆市现代中药制药工程技术研究中心,400054 [4]重庆医疗器械质量检验中心,401121

出　　处：《中国执业药师》2011年第8期28-29,共2页China Licensed Pharmacist

基　　金：国家科技重大专项课题(2010ZX09401-306-2-23);重庆市科委产业化项目(CSTC;2008AB5116)

摘　　要：目的:建立测定麦冬中沿阶草酮甲的高效液相色谱法。方法:色谱柱:Hypersil C18柱(150mm×4.6mm,5μm);流动相:甲醇-水(75∶25);流速1.0mL/min;检测波长298nm。结果:沿阶草酮甲进样量在0.918～5.508μg范围内与峰面积线性关系良好,回归方程Y=3645282X-420010(r=0.99976),平均回收率为99.80%,RSD=1.74%(n=6)。结论:所建立的高效液相色谱法简便、快速、重复性好,可用于麦冬中沿阶草酮甲的质量控制。Objective：To establish HPLC method for the content determination of ophiopogonanone A in Radix Ophiopogonis.Methods：The analysis was carried out on Hypersil C18 column （150 mm × 4.6 mm,5 μm）.The mobile phase was MeOH-water（75∶25） with flow-rate of 1.0 mL/min.The eluate was detected at 298 nm in room temperature.Results：The method was simple and had a good linear relationship.The linear range of ophiopogonanone A was 0.918 ～ 5.508 μg （r = 0.999 76）.The recovery was 99.80% （RSD = 1.74%）.Conclusion：The method was simple and suitable for the content determination of ophiopogonanone A in Radix Ophiopogonis.

关 键 词：高效液相色谱 麦冬 沿阶草酮甲 含量测定 

分 类 号：R286.0[医药卫生—中药学]