大豆品种合丰25体细胞胚发生条件的优化  

Optimizing of Regeneration Conditions of Somatic Embryo for Soybean Variety "Hefeng 25"

作　　者：李宁辉[1] 徐鹏飞[1] 范素杰[1] 吴俊江[2] 李文滨[1] 王萍[3] 王欣[1] 姜良宇[1] 王金生[2] 张淑珍[1] 

机构地区：[1]东北农业大学大豆研究所大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [3]淮海工学院海洋学院,江苏连云港222005

出　　处：《作物杂志》2011年第4期23-28,共6页Crops

基　　金：教育部新世纪人才培养计划项目(NCET-09-164);国家自然科学基金(30972889;31071439);黑龙江省自然科学基金(C200814);黑龙江省新世纪优秀人才培养计划项目(NCET-06-007);中国博士后基金(20060400835);黑龙江省博士后基金(LRB06-010);博士后落户黑龙江科研启动基金资助项目(LBH-Q05032)

摘　　要：为优化大豆体细胞胚再生植株的条件,提高大豆遗传转化的效率,本研究选用大豆品种合丰25为试验材料,针对影响体细胞胚诱导的3个关键因素pH值、2,4-D浓度及光照条件进行体细胞胚发生频率的研究。结果表明:pH值、2,4-D浓度、光照条件这3种因素的不同处理对体细胞胚的诱导影响显著,其幼胚的发生频率最高为70.70%、最低为3.70%。当pH值为7.0、2,4-D浓度为20mg/L,黑暗培养最有利于合丰25体细胞胚的诱导,其胚的发生频率为70.70%,明显高于其他处理。To optimize regeneration conditions of soybean somatic embryo and to improve efficiency of soybean genetic transformation,the pH value,2,4-D concentration and illumination condition were studied using ＂Hefeng 25＂ as the test material.The results showed that the different treatments of the three factors had a significant influence on the development of somatic embryo.The highest efficiency was 70.70%,while the lowest efficiency was 3.70%.When the pH value was 7.0,2,4-D concentration was 20mg/L and the materials were cultured in dark,the efficiency of soybean somatic embryo could reach 70.70%,much higher than that of other treatments.

关 键 词：合丰25 体细胞胚 大豆再生 优化 

分 类 号：S565.1[农业科学—作物学]