HPLC-ELSD法测定艾可清胶囊中毛冬青皂苷甲的含量  

HPLC-ELSD Determination of Ilexsaponin A in Aikeqing Capsules

作　　者：卢元媛[1] 张玉虎[1] 黄建昌[1] 刘抗伦[1] 符林春[1] 胡英杰[1] 

机构地区：[1]广州中医药大学热带医学研究所药学研究室,广州510405

出　　处：《中国实验方剂学杂志》2011年第17期62-65,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项(2008ZX10005-005;2009ZX09103-414);广东省科技计划项目(2007A050100008);科技部国际合作项目(2008DFA32320)

摘　　要：目的:建立艾可清胶囊中毛冬青皂苷甲的硅胶薄层色谱(TLC)鉴别方法和高效液相色谱-蒸发光散射(HPLC-ELSD)检测的含量测定方法。方法:自药材中分离鉴定毛冬青皂苷甲;确定TLC条件;采用ODS色谱柱(4.6 mm×250 mm,5μm)及保护柱,以甲醇-0.2%冰醋酸(67∶33)为流动相,ELSD检测器,漂移管温度95℃,载气流速2.5 L.min-1,柱温30℃,流速1.0 mL.min-1作为HPLC条件。结果:设立的TLC条件可以从复方制剂中检出毛冬青皂苷甲;建立的HPLC含量测定方法,毛冬青皂苷甲在1.92～9.60μg呈良好线性关系(r=0.999 5),平均回收率为99.85%,RSD 1.04%。结论:建立的毛冬青皂苷甲的硅胶TLC鉴别方法和HPLC-ELSD含量测定方法具有操作快捷、方法准确的特点,可作为毛冬青药材及其复方制剂的质量控制方法。Objective：To establish a quality control method for identification and assay of ilexsaponin A for Aikeqing Capsules,a compound Chinese materia medica Radix Ilicis Pubescentis containing.Method： Ilexsaponin A was isolated and identified from the roots of Ilex pubescens.The Phenomenex Kromasil C18（4.6 mm×250 mm,5 μm） column with a Phenomenex Security Guard C18 guard column was used with methanol-0.2% acetic acid（67∶33） as the mobile phase at a flow rate of 1.0 mL·min-1.The drift tube temperature of ELSD was set at 95 ℃,the gas flow was at 2.5 L·min-1.Result： Ilexsaponin A was identified by silica gel TLC from the compound preparation of Chinese materia medica.The linear range of ilexsaponin A was 1.92-9.60 μg（r=0.999 5）,and its average recovery was 99.85% with RSD 1.04%.Conclusion： The methods established are rapid and accurate,and can be used for quality control on Radix Ilicis Pubescentis or its compound preparation.

关 键 词：艾可清胶囊 毛冬青皂苷甲 薄层色谱 高效液相色谱-蒸发光散射 

分 类 号：R284.1[医药卫生—中药学]