大鼠颈总动脉粥样硬化易损斑块模型建立与评价  

Establishment and Evaluation of Vulnerable Atherosclerotic Plaque in Carotid Artery in Rats

作　　者：王建辉[1] 李磊[1] 柳芳[1] 史跃[1] 马彦雷[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中国实验方剂学杂志》2011年第17期158-162,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073085);国家科技重大专项;重大新药创制项目(2009zx09301-005;2009zx09303-003)

摘　　要：目的:观察三因素不同组合对大鼠颈总动脉粥样硬化斑块稳定性的影响。方法:采用三因素,即高脂、高蛋氨酸及球囊拉伤术,通过不同组合建立4个模型组,分别为高脂结合球囊拉伤术组,高脂结合高蛋氨酸组,高蛋氨酸结合球囊拉伤术组,高脂、高蛋氨酸结合球囊拉伤术组。各模型组给予相应饲料喂养,第4周后各球囊拉伤术组行颈总动脉球囊拉伤术,12周末测定血脂、血浆同型半胱氨酸(Hcy)、血清高敏C-反应蛋白(hs-CRP)水平及病理形态学相关指标对4个模型组进行评价。结果:与对照组比较,高脂+高蛋氨酸结合球囊拉伤术组(三因素组)血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C),血浆Hcy、血清hs-CRP水平均有显著升高(P<0.01)。各模型组间比较,三因素组斑块面积以及管腔狭窄率明显。结论:高脂、高蛋氨酸结合球囊拉伤术组颈总动脉粥样硬化斑块形成较其他模型组明显,血清hs-CRP升高显著,提示粥样硬化斑块不稳定倾向,表明高脂血症、高同型半胱氨酸血症(HHcy)、球囊拉伤术造成内皮损伤三者有协同作用,共同促进颈总动脉粥样硬化进展及易损斑块形成。Objective：To observe the effects of three factors with different combination on stability of vulnerable atherosclerotic plaque of carotid artery in rats.Method： With three factors,high lipid,2% L-methionine and carotid artery balloon injury,we established four models of vulnerable atherosclerotic plaque of carotid artery with their different combination,they were high lipid with carotid artery balloon injury,high lipid with 2%L-methionine,2%L-methionine with carotid artery balloon injury,and high lipid,2%L-methionine with carotid artery balloon injury（three factors model group）.Each model group was given the relevant feed,and carotid artery balloon injury was operated at the fourth week.After 12 weeks,the levels of lipid,Hcy,hs-CRP and some plaque indexes were measured to evaluate four models.Result： In contrast to control group,the levels of TC,LDL-C,Hcy,and hs-CRP in three factors model group were raised significantly（P0.01）.In contrast to the others,the plague area related indexes（PA,PA/IEL-A,PA/EEM-A） of three factors model group were obvious.Conclusion： The atherosclerotic plaque of carotid artery in three factors group was obvious,the level of hs-CRP was raised,it showed that the plaque tended to rupture,and indicated there was synergistic effect among hyperlipemia,hyperhomocysteine（HHcy）,and vascular endothelial dysfunction.They worked together to make the formation and progress of the atherosclerotic vulnerable plaque of carotid artery.

关 键 词：易损斑块模型 高同型半胱氨酸血症 高敏C-反应蛋白 

分 类 号：R285.5[医药卫生—中药学]