化瘀丸对小鼠Lewis肺癌生长及血栓形成相关因子的干预作用  

Effect of Huayu Wan on Tumor Growth and Expression of Thrombosis Related Factors in Lewis Lung Cancer-bearing Mice

作　　者：于明薇[1] 杨国旺[1] 王笑民[1] 

机构地区：[1]首都医科大学附属北京中医医院肿瘤科,北京100010

出　　处：《中国实验方剂学杂志》2011年第17期163-166,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市卫生局;北京市中医管理局青年科学研究资助项目(QN2009-11)

摘　　要：目的:观察以益气活血解毒为治法的化瘀丸对肿瘤生长及血栓形成相关因子的影响。方法:建立小鼠Lewis肺癌动物模型,造模后24 h随机分组并开始给药,实验分为5组:模型组(无菌水10 mL.kg-1 ig,0.9%NaCl 10 mL.kg-1 ip,1次/d)、低分子肝素组(无菌水10 mL.kg-1 ig,低分子肝素注射液100 u.kg-1 i,p)、环磷酰胺(CTX)组[无菌水10 mL.kg-1 ig,CTX50 mg.kg-1(1,61,1,16 d)ip,1次/d]、化瘀丸组(化瘀丸4 g.kg-1 ig,0.9%NaCl 10 mL.kg-1 ip,1次/d)、空白对照组(无菌水10 mL.kg-1 ig,0.9%NaCl 10 mL.kg-1 ip,1次/d),给药12 d或19 d。定期观察各组小鼠体重及肿瘤生长情况,接种后132,0 d处死小鼠,检测瘤重、肺转移数,酶联免疫法(ELISA)检测小鼠血清肿瘤坏死因子-α(TNF-α)、P-选择素(P-selectin)、内皮素-1(ET-1)水平。结果:化瘀丸组小鼠瘤重显著低于模型组(P<0.01),抑瘤率分别为34.97%(13 d)和31.52%(20 d),肺转移抑制率为83.25%(20 d)。20 d化瘀丸组小鼠血清ET-1水平低于模型组(P<0.05),P-selectin,ET-1水平低于低分子肝素组(P<0.05),并显著低于CTX组(P<0.01)。结论:化瘀丸作用于Lewis肺癌荷瘤小鼠,具有一定抗肿瘤生长的作用,并能降低荷瘤小鼠异常升高的血栓形成相关因子水平。Objective：To observe the effect of Huayu Wan on tumor growth and expression of thrombosis related factors in Lewis lung cancer-bearing mice.Method： Lewis lung cancer model in mice was established.They were then divided into 5 groups 24 h after model establishment：model group（sterile water 10 mL·kg-1 ig,0.9%NaCl 10 mL·kg-1 ip,daily）,low-molecular-weight heparins group（sterile water 10 mL·kg-1 ig,low-molecular-weight heparins 100 u·kg-1 ip,daily）,cyclophosphamide（CTX） group（sterile water 10 mL·kg-1 ig,CTX 50 mg·kg-1（1,6,11,16 d） ip,once）,Huayu Wan group（Huayu Wan 4 g·kg-1 ig,0.9% NaCl 10 mL·kg-1 ip,daily） and control group（sterile water 10 mL·kg-1 ig,0.9%NaCl 10 mL·kg-1 ip,daily）.The tumor inhibiting rate,the inhibiting rate of lung metastasis and change of weight was observed regularly and the levels of tumor necrosis factor-α（TNF-α）,P-selectin,endothelin-1（ET-1） in peripheral blood serum were tested by ELISA.Result： The decrease of tumor weight was obvious in Huayu Wan group（P0.01）.The tumor inhibition rate was 34.97%（13 d） and 31.52%（20 d）,the inhibiting rate of lung metastasis was 83.25%（20 d）.The level of ET-1 in peripheral blood serum of Huayu Wan group was lower than those in model group（P0.05）.The level of P-selectin and ET-1 in peripheral blood serum of Huayu Wan group were lower than those in low-molecular-weight heparins group（P0.05）,the decrease was obvious compared with CTX group（P0.01）.Conclusion： Huayu Wan can be an effective measure for inhibiting the tumor growth and reducing the expression of thrombosis related factor in lewis lung cancer-bearing mice.

关 键 词：益气活血解毒 肿瘤 血栓形成 化瘀丸 

分 类 号：R285.5[医药卫生—中药学]