大鼠慢性浅表性胃炎模型的建立  

Rat Model of Chronic Superficial Gastritis

作　　者：任守忠[1] 马志健[2] 王继浩[3] 曾贵荣 

机构地区：[1]海南医学院药学院,海口571101 [2]海南医学院解剖教研室,海口571101 [3]海南医学院中医学院,海口571101

出　　处：《中国实验方剂学杂志》2011年第17期191-194,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:采用综合法建立大鼠慢性浅表性胃炎(CSG)动物模型。方法:应用60%乙醇,20 mmol.L-1去氧胆酸钠和0.05%～0.1%氨水刺激胃黏膜,并结合饥饱失常。12周后观察大鼠胃黏膜病理变化及胃液酸度、胃蛋白酶活性和游离黏液量等生化指标变化。结果:与对照组比,模型组动物一般情况差、体重增加缓慢,胃窦黏膜炎性细胞浸润,炎症评分明显增加;游离酸和总酸度明显增加,游离黏液量和胃蛋白酶活性明显升高。结论:应用60%乙醇和20 mmol.L-1去氧胆酸钠,0.05%～0.1%氨水并结合饥饱失常对SD大鼠刺激12周可成功地建立CSG模型,且稳定性好。Objective：To establish a rat model of chronic superficial gastritis.Method：The experimental SD rats were given 60% alcohol and 20 mmol·L-1 sodium deoxycholate,0.05%-0.1% ammonia water and also treated with irregular diet for 12 weeks,pathologic changes in gastric mucosa were investigated by microscope.Biochemical indexes were measured with instruments.Result： Compared with the control group,the body weight in the model group decreased obviously,the concentration of free acid and total acid were significant increased,free mucin content and pepsin activity were significant higher.Conclusion： The above described method could establish rat model of chronic superficial gastritis successfully.

关 键 词：浅表性胃炎 动物模型 乙醇 去氧胆酸钠 氨水 

分 类 号：R285.5[医药卫生—中药学]