溃疡平对乙酸性胃溃疡大鼠胃黏膜血流量及血清ET-1,NO含量的影响  

Effects of Chinese Medicine Kuiyangping on Gastric Mucosal Blood Flow and the Serum Concentration of ET-1 and NO in Rats with Acetic Acid-induced Gastric Ulcer

作　　者：赵红晔[1] 曹军[2] 王月飞[1] 赫灵菊 周丽[1] 卢长柱[1] 王滨[1] 

机构地区：[1]齐齐哈尔医学院生理教研室,黑龙江齐齐哈尔161006 [2]中国中医科学院中药研究所,北京100700 [3]齐齐哈尔市富拉尔基区中医医院,黑龙江齐齐哈尔161041

出　　处：《中国实验方剂学杂志》2011年第17期213-215,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省中医管理局项目(ZHY10-Z107)

摘　　要：目的:观察溃疡平对乙酸性胃溃疡大鼠胃黏膜血流量(GMBF)及血清内皮素-1(ET-1)和一氧化氮(NO)含量的影响。方法:采用改良Okabe法制备大鼠慢性乙酸胃溃疡模型,随机分为:正常组、模型组、溃疡平低、中、高剂量组(1.40,2.80,5.60 g.kg-1 i,g)和盐酸雷尼替丁组(2.83×10-2 g.kg-1 i,g)6组。给药14 d后,运用MP150多道生理记录系统中的LDF100C激光多普勒血流测定模块测定溃疡周边部的GMBF;ELISA法检测血清ET-1的含量;硝酸盐还原酶法检测血清NO含量。结果:与模型组相比,溃疡平高剂量组GMBF明显增多(P<0.05),血清ET-1含量明显降低(P<0.01),血清NO含量明显升高(P<0.05);与盐酸雷尼替丁组相比,溃疡平高剂量组GMBF明显增多(P<0.05),血清ET-1含量明显降低(P<0.01),血清NO含量明显升高(P<0.05)。结论:溃疡平刺激NO的合成和释放,反馈性抑制ET-1的释放,从而增加GMBF,这可能是其加速胃溃疡愈合和改善溃疡愈合质量(QOUH)的作用机制之一。Objective：To investigate the effect of traditional chinese medicine Kuiyangping on gastric mucosal blood flow（GMBF） and the serum concentration of endothelin-1（ET-1） and nitric oxide（NO） rats with in acetic acid-induced gastric ulcer（GU）.Method：The ulcer model in rats was prepared with Okabe＇s method with some modifications.The rats were randomly divided into 6 groups：normal control group,model group,low,middle and high-dose of Kuiyangping groups（1.4,2.8,5.6 g·kg-1,ig） and ranitidine hydrochloride group（2.83×10-2g·kg-1,ig）.After the 14 days treatment,GMBF of the mucosa around the GU were measured by the LDF100C of MP150 BIOPAC System.We also determined the serum concentration of ET-1 by ELISA,and determined the serum concentration of NO by nitrate reductase one step method.Result：Compared with model group,GMBF was higher（P0.05）,the serum concentration of ET-1 was lower（P0.01） and NO was higher（P0.05） significantly in high-dose of Kuiyangping group.Compared with ranitidine hydrochloride group,GMBF was higher（P0.05）,the serum concentration of ET-1 was lower（P0.01） and NO was higher（P0.05） significantly in high-dose of Kuiyangping group.Conclusion：Kuiyangping could promote the synthesis and release of NO,however,NO could inhibit the release of ET-1.So Kuiyangping could increase the GMBF.Which may be one of the mechanisms of Kuiyangping to promote the healing of gastric ulceration and improve quality of ulcer healing.

关 键 词：溃疡平 胃黏膜血流 内皮素-1 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]