蟾酥对豚鼠离体心脏的毒性作用和物质基础研究  

Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart

作　　者：蒋洁君[1] 周婧[1] 马宏跃[1] 尤奋强[2] 段金廒[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省方剂研究重点实验室,南京210046 [2]南京中医药大学昆山附属医院,江苏昆山215300

出　　处：《中国实验方剂学杂志》2011年第17期233-237,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学青年基金(30901894);教育部新教师基金(20093237120013);霍英东教育基金会第十二届高等院校青年教师基金(121044);江苏省中医药局项目(LZ09017);南京中医药大学青年自然基金(09XZR21)

摘　　要：目的:研究蟾酥对豚鼠离体心脏的毒性作用和其物质基础。方法:通过Langendorff灌流研究蟾酥对豚鼠离体心脏的毒性作用,HPLC测定灌流后心脏中蟾蜍甾烯的含量。结果:蟾酥诱导豚鼠离体心脏出现房室传导阻滞、室速、室颤多种心律失常现象。当给药量累积达(60±11.5)μg时致其停跳。采用HPLC在灌流心脏中测到9种蟾蜍甾烯类化合物,其与心脏亲和力增加的顺序为:沙蟾毒精>蟾毒灵>远华蟾毒配基>嚏根草苷元>日蟾毒它灵>华蟾毒灵>蟾毒它灵>脂蟾毒配基>去乙酰蟾毒它灵。结论:蟾酥对豚鼠心脏具有显著毒性,其物质基础可能是脂蟾毒配基、蟾毒灵、蟾毒它灵、日蟾毒灵等9种蟾蜍甾烯类化合物。Objective：To study toad venom（BV）-induced cardiac toxicity and the toxic substances in isolated guinea-pig heart.Method：Isolated guinea pig hearts were switched to the Langendorff mode and perfused with or without BV.Measure the content of toad venom in perfused hearts using HPLC.Result：BV caused auriculo-ventricular block,supraventicular tachycardia,ventricular tachycardia,ventricular fibrillation and other arrhythmias phenomenon in isolated hearts.The cumulative doses of bufadienolides（60±11.5）μg induced cardiac arrest.Nine kinds of bufadienolides were detected by HPLC in the isolated hearts.Compare their affinity with the hearts：arenobufaginbufalintelocinobufaginhellebrigeningamabufotalincinobufalinbufotalinresibufogenindesacety-bufotalin.Conclusion：BV has cardiotoxicity on the guinea pig heart and the material basis of toad induced toxic effects may be gamabufotalin,arenobufagin and so on.

关 键 词：蟾酥 心脏毒性 离体心脏灌流 HPLC 物质基础 

分 类 号：R284.1[医药卫生—中药学]