酶促DNA合成研究的进展  

Advance in Enzymatic DNA Synthesis

作　　者：向义和[1] 

机构地区：[1]清华大学物理系

出　　处：《自然杂志》2011年第4期231-238,共8页Chinese Journal of Nature

摘　　要：笔者介绍了酶促DNA合成研究的进展。科恩伯格和他的同事经历了从合成核苷酸、核苷三磷酸到合成DNA的历程。他们分离并提纯了DNA聚合酶,弄清了合成DNA的最直接的前体,揭示了DNA合成的化学机理,合成了具有感染性的噬菌体ΦX174DNA。The advance in enzymatic DNA synthesis is introduced. Kornberg and his colleagues went through deoxyribonucleotide, deoxynucleoside tryphosphates and DNA synthesis. The immediate precursor of DNA synthesis was known. DNA polymerase was separated and purified. The chemical mechanism of DNA synthesis was revealed and infectious phage ФX174DNA was synthesized.

关 键 词：核苷三磷酸 DNA聚合酶 酶的纯化 噬菌体ΦX174DNA 复制型 密度梯度离心 

分 类 号：Q523[生物学—生物化学]