实验设计在优化水稻愈伤培养基中的应用  

Application of Experiment Designation in Optimizing Callus Medium of Rice

作　　者：陈雪能[1] 黄群策[1] 张树寅[1] 赵帅鹏[1] 周莎莎[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《种子》2011年第8期26-28,33,共4页Seed

基　　金：河南省科技前沿基础研究项目(82300433202)

摘　　要：以同源四倍体双胚苗水稻DER 10-04-01成熟种子为试验材料进行无性系诱导,统计愈伤组织的诱导率、平均愈伤指数和愈伤组织的生长率。运用田口设计方法分析培养基中2,4-D,NAA和NOA不同的浓度配比对愈伤组织诱导率、生长率和平均愈伤指数的影响,由此获得最适于试验材料愈伤组织诱导和培养的优化组合。试验结果表明,2,4-D,NAA和NOA均对愈伤组织的诱导率、平均愈伤指数和生长率有一定的影响,其最优培养组合(MS+2,4-D 2 mg/L+NAA2 mg/L+NOA 3 mg/L)在无性系诱导和培养中有一定的参考价值。The twin-embryos seed of autotetraploid rice DER 10-04-01 was taken as the inducs material, and analyzed initiation rate, average callus index and growth rate. The effect of different concentration and ratio of 2,4-D, NAA and NOA able tetraploid double on callus induction rate, growth rate and the average callus index so as to the most suitembryo seedlings of DER 10-04-01 rice was analysed. Experiments showed that： rate, average, callus index and growth for the tetraploid double embryo rate were all effected by 2,4-D, NAA and NOA , and the best combination seedlings DER 10-04-01 rice （MS ＋ 2,4-D 2 mg/L ＋ NAA 2 mg/L ＋ NOA 3 mg/L）could guide cultures for clone induction .

关 键 词：田口设计 生长率 愈伤组织 培养基优化 

分 类 号：S511[农业科学—作物学]