壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠产生的免疫反应  

The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier

作　　者：蒋金凤[1,2] 程安春[1,3,2] 汪铭书[1,3,2] 路立婷[1,2] 朱德康[3,2] 贾仁勇[1,3,2] 陈孝跃[3,2] 

机构地区：[1]四川农业大学预防兽医研究所,成都611130 [2]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,成都611130 [3]四川农业大学动物医学院禽病防治研究中心,四川雅安625014

出　　处：《中国农业科学》2011年第16期3454-3462,共9页Scientia Agricultura Sinica

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);现代农业产业技术体系建设专项资金(CARS-43-8);"973"计划(2011CB111606);国家自然科学基金(31072157)

摘　　要：【目的】探讨壳聚糖对鸭肠炎病毒(duck enteritis virus,DEV)gC基因疫苗免疫反应的影响。【方法】应用壳聚糖作为DEV gC基因疫苗(pcDNA-DEV-gC)递送载体,采用肌肉注射和口服单次免疫Balb/c小鼠50μg剂量基因疫苗,并以肌注不同剂量裸质粒组、弱毒组和生理盐水组作为对照,分别检测不同疫苗免疫后的细胞、体液和黏膜免疫水平。【结果】肌注壳聚糖疫苗组诱导小鼠产生较肌注裸质粒50μg组和口服壳聚糖疫苗组高的细胞和体液免疫反应,且产生与弱毒疫苗相似的细胞免疫应答,但弱毒疫苗诱导体液免疫的能力更强,仅口服壳聚糖疫苗组产生粘膜免疫。【结论】上述研究结果表明壳聚糖具有一定的佐剂效应,为获得更有效的DEV gC基因疫苗提供了新策略,但该疫苗的推广应用还有待于对影响疫苗免疫效果的各因素进行进一步优化。[Objective ] The objective of the experiment is to investigate the influence of chitosan on the immunoreaction induced duck enteritis virus gC genetic vaccine. [Method] Balb/c mice were immunized with 501xg per dose ofDEV gC genetic vaccine with chitosan as deliver carrier by a single intramuscular injection and oral. Naked plasmid groups of different immunizing doses by intramscular injection, live attenuated vaccine group and physiological saline group were applied as control groups. Cellular immunity, humora} immunity and mucosal immunity were detected, respectively. [Result] Compared with DEV gC genetic vaccine with ehitosan as deliver carrier by oral and 50 μg of naked plasmid group by intramuscular injection, DEV gC genetic vaccine with chitosan as deliver carrier by intramuscular injection induced stronger cellular and humoral immunity. This vaccine induced similar cellular immune response compared with live attenuated vaccine that induced stronger humoral immune responses. Mucosal immunity was induced only by oral DEV gC genetic vaccine with chitosan as deliver carrier. [Conclusion] Chitosan had certain adjuvant＇s ability. It has provided a new strategy to obstain more effective DEV gC genetic vaccine. Many factors affecting the immunity efficiency of vaccine need to be optimiazed further for application of this vaccine generally,

关 键 词：壳聚糖 DEVgC基因 基因疫苗 细胞免疫 体液免疫 粘膜免疫 

分 类 号：S858.32[农业科学—临床兽医学]