金雀异黄酮对体外培养缺氧大鼠骨髓间充质干细胞增殖与成骨分化的影响  

Effect of Genistein on Proliferation and Differentiation of Rat Bone Mesenchymal Stem Cells Treated with Hypoxia in Vitro

作　　者：韩桂秋[1] 葛宝丰[1] 陈克明[1] 马慧萍[2] 

机构地区：[1]兰州军区兰州总医院骨科研究所,兰州730050 [2]兰州军区兰州总医院药材科,兰州730050

出　　处：《中国药学杂志》2011年第16期1248-1253,共6页Chinese Pharmaceutical Journal

基　　金：甘肃省科技重大专项计划资助项目(092nkDA025)

摘　　要：目的观察金雀异黄酮对缺氧体外培养大鼠骨髓间充质干细胞(BMSCs)增殖与成骨分化的影响。方法采用贴壁筛选法体外培养BMSCs并用三气培养箱建立缺氧模型。设常氧对照组、缺氧对照组和缺氧加药组,其中缺氧加药组细分为1×10-8、1×10-7和1×10-6 mol.L-1组金雀异黄酮组,缺氧处理36 h后分析各组的细胞增殖和成骨性指标包括碱性磷酸酶(ALP)活性、Ⅰ型胶原表达、钙盐沉积量和钙化结节面积等,并用Real Time RT-PCR法检测Runx2和骨形成蛋白(BMP)-2的基因表达情况。结果与缺氧对照组比较,金雀异黄酮抑制BMSCs增殖,显著提高ALP活性、Ⅰ型胶原表达量、钙盐沉积量和钙化结节面积,并提高基因Runx2和BMP-2的表达水平,且呈现出剂量依赖性特点。结论金雀异黄酮具有抑制缺氧BMSCs增殖并促进其成骨分化的作用。OBJECTIVE To investigate the effects of genistein on proliferation and osteogenic differentiati of rat bone mesenchymal stem eells（BMSCs） treated with hypoxia. METHODS Rat BMSCs were cultured by adherence screening method and hypoxie environment was made by triple-gases incubator. At 36 hours after hypoxia, proliferation and osteogenic differentiation markers including alkaline phosphatase （ALP） activity ,collagen I ,calcium salt sediment yield and mineralized bone nodules were compared among 1 ×10^-8, 1 × 10^-7, 1 × 10^-6 mol · L^- 1 group,Hypoxia Control and Normal Control. Total RNA was isolated and the gene expression of Runx2 and bone morphogenetic proteins-2 （BMP-2） was investigated by Real Time RT-PCR. RESULTS Genistein decreased proliferation and improved ALP activity, expression of collagen I , calcium Salt sediment yield and area of mineralized nodules significantly. Besides, the relative level of Runx2 and BMP-2 mRNA expression were enhanced. CONCLUSION Genistein can inhibited proliferation and enhance osteogenic differentiation of BMSCs treat with hvpoxia significantlv.

关 键 词：金雀异黄酮 骨髓间充质干细胞 缺氧 成骨性分化 增殖 I型胶原 碱性磷酸酶 核心结合因子A1 骨形态蛋白-2 

分 类 号：R285.5[医药卫生—中药学]