HPLC-PAD测定刺五加中刺五加苷B与刺五加苷E的含量  

Determination of Eleutheroside B and Eleutheroside E in Acanthopanax senticosus by HPLC-PAD

作　　者：朱燕燕[1] 尉小慧[1,2,3,4] 吴弢[1,2,3,4] 黎万奎[1,2,3,4] 王峥涛[1,2,3,4] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201210 [2]上海中医药大学中药研究所上海中药标准化研究中心,上海201210 [3]上海中医药大学中药研究所中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201210 [4]上海中医药大学中药研究所上海市复方中药重点实验室,上海201210

出　　处：《中国药学杂志》2011年第16期1280-1282,共3页Chinese Pharmaceutical Journal

基　　金：上海市科学技术委员会资助项目(07DZ19727);上海市教委创新团队建设

摘　　要：目的研究HPLC同时测定刺五加中刺五加苷B与刺五加苷E的含量。方法采用Agilent Zorbax SB-C18柱(4.6mm×250 mm,5μm),流动相为乙腈-0.5%磷酸(0～10 min,9∶91;10～30 min,9～20∶91～80),流速1.0 mL.min-1,检测波长220 nm,柱温25℃。结果刺五加苷B、刺五加苷E分别在0.35～34.83与0.69～69.20μg.mL-1内线性关系良好,其相关系数分别为0.999 9与1.000 0;精密度RSD均为0.4%,小于2%;重复性RSD分别为1.4%与1.0%,稳定性RSD分别为3.1%与3.4%,均小于5%;平均回收率(n=9)分别为97.4%(RSD为5.5%)和102.7%(RSD为4.3%)。结论本方法操作简便,结果可靠,重复性好,可作为刺五加及其制剂的质量控制方法。OBJECTIVE To develope a HPLC method for quantitative determination of eleutheroside B and eleutheroside E in Acanthopanax senticosus. METHODS The separation of eleutheroside B and elentheroside E was performed on an Agilent Zorbax SB- C18 column （4. 6 mm × 250 mm,5 μm） by gradient elution with 0. 5% aqueous phosphoric acid and acetonitrile as the mobile phase. The flow rate was 1.0 mL · min- 1 at 25 ℃, and the detection wavelength was set at 220 nm. RESULTS The linear ranges of eleutheroside B and eleutheroside E were 0. 35 - 34. 83 and 0. 69 - 69. 20 μg . mL-1, respectively, and the correlation coefficient were 0. 999 9 and 1. 000 0, respectively. The variations for intra- and inter-day precision were less than 3.1% and 3.4% ,and the accuracy （ n = 9） for eleuthereside B and eleutheroside E were 97.4% （ RSD = 5.5% ） and 102. 7% （ RSD = 4. 3% ）, respectively. CONCLUSION This method is simple, accurate,reliable and reproducible for the quality control of Acanthopanax senticosus.

关 键 词：刺五加 刺五加苷B 刺五加苷E 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]