水稻不育系的广亲和基因检测及籼粳型分析  

Identification of S5-n gene and subspecies classification of indica and japonica in rice male sterile line

作　　者：夏士健[1,2] 张启军[1,2] 杨杰[1,2] 吕川根[1,2] 

机构地区：[1]江苏省农业科学院粮食作物研究所 [2]国家水稻改良中心南京分中心,江苏南京210014

出　　处：《江苏农业学报》2011年第4期698-703,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家"863"计划(2006AA100101)资助项目

摘　　要：为鉴定部分水稻不育系的广亲和性和籼粳型,根据籼粳亚种间广亲和基因S5-n在第6染色体存在136 bp片段缺失的特征,设计InDel标记S5136,对培矮64S、广占63S等不育系进行广亲和基因鉴定。利用与栽培稻籼、粳遗传分化密切相关的34个InDel标记,对这些不育系PCR产物的电泳结果进行判读和分析,计算其籼型或粳型基因频率(InDel分子指数法)。结果表明:①三系不育系粤泰A具有广亲和基因S5-n。②两系不育系培矮64S、N111S、C815S等11个不育系具有广亲和基因S5-n。③目前广泛应用于生产的不育系培矮64S、广占63S、Y58S等的籼型基因频率均为0.76～0.91,据此认为具有10%～25%左右粳稻血缘的籼型不育系可能更有利于亚种间杂种优势利用。According to the 136 bp deletion of wide compatibility gene（S5-n） in comparison with alleles in indica and japonica varieties at S5 locus,S5136,an InDel marker was designed to identify S5-n gene in rice male sterile lines,Peiai64S,Guangzhan63S,etc.With 34 InDel markers which related closely to genetic differentiation of rice indica or japonica variety,male sterile lines were analyzed for their indica or japonica gene frequency（InDel molecular method） by gel electrophoresis of PCR product.The results showed that： ① A cytoplasm male sterility line,YuetaiA,possessed wide compatibility gene,S5-n.② Ten dual-purpose genic male sterile lines,such as Peiai64S,N111S,and C815S etc.possessed S5-n gene.③ Peiai64S,Guangzhan63S,Y58S,etc.which were widely used in the present rice production,displayed their indica gene frequency in a range of 0.76-0.91,from which it could be suggested that rice male sterile lines holding genes with 10%-25% of indica type might be more favorable for the utilization of heterosis of hybrid rice.

关 键 词：水稻 不育系 广亲和基因S5-n InDel分子指数法 

分 类 号：S511.035[农业科学—作物学]