中药复方透皮贴剂对奶牛临床型乳房炎的疗效及其机理  

Curative effect of transdermal patch made of compound traditional Chinese medicine on clinical mastitis of dairy cows and its mechanism

作　　者：冯士彬[1] 李志明[2] 孟庆娟[1] 王希春[1] 韩春杨[1] 邹松阳[2] 刘琦山[2] 吴金节[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽白帝乳业有限公司,安徽合肥230031

出　　处：《江苏农业学报》2011年第4期790-794,共5页Jiangsu Journal of Agricultural Sciences

基　　金：安徽省科技攻关项目(08010302157)

摘　　要：为了探明中药复方透皮贴剂治疗奶牛临床型乳房炎的效果及机理,本研究选取85头患有临床型乳房炎的荷斯坦奶牛,随机分成3组,分别采用中药复方透皮贴剂、青链霉素及贴剂与青链霉素联合用药进行临床治疗,并测定中药复方透皮贴剂和青链霉素组奶牛治疗前后血液流变学指标和血清中的细胞因子含量,与10头健康奶牛进行比较,观察中药复方透皮贴剂的疗效。结果发现:中药复方透皮贴剂、青链霉素、贴剂与青链霉素联合用药对奶牛临床型乳房炎的治愈率分别为88.24%、80.56%和94.12%,有效率分别为94.12%、91.67%和100.00%,以贴剂与青链霉素联合用药治疗效果最佳;临床型乳房炎奶牛治疗前120 S-、70 S-1、50 S-1、30 S-1等切变率下全血粘度和全血还原粘度、红细胞变形指数、红细胞聚积指数、红细胞刚性指数等血液流变学指标和血清中IL-1β、IL-8、TNF-α水平均显著高于健康奶牛(P<0.01或P<0.05),治疗7 d后上述指标均显著降低(P<0.01或P<0.05),与健康奶牛相比差异不显著(P>0.05)。试验证实了临床型乳房炎奶牛"血瘀证"的客观存在;血液流变学指标和血清中IL-1β、IL-8、TNF-α可以用作乳房炎诊断和治疗效果的评价;中药复方透皮贴剂对奶牛临床型乳房炎有较好的治疗效果,能够改善患病奶牛微循环、抑制炎症反应、降低血液粘度和炎性细胞因子的含量。In order to investigate the curative effect and its mechanism of transdermal patch made of compound traditional Chinese medicine,85 dairy cows with clinical mastitis were selected and divided into three groups which were respectively treated with transdermal patch made of compound traditional Chinese medicine,penicillin and streptomycin,combination of transdermal patch with penicillin and streptomycin.The hemorrheologic indices and serum cytokine levels of dairy cows with clinical mastitis before and after treatment.Meanwhile,the hemorrheologic indices and serum cytokine levels of 10 healthy dairy cows were determined as well.The results showed that the curative rate of clinical mastitis by transdermal patch made of compound traditional Chinese medicine,penicillin and streptomycin,combination of transdermal patch with penicillin and streptomycin were 88.24%,80.56%,and 94.12%,respectively,and the effective rate were 94.12%,91.67% and 100%,respectively.The curative effect of drug combination of transdermal patch with penicillin and streptomycin was better than others.Whole blood viscosity,whole blood reduced viscosity at shear rates of 120 S-1,70 S-1,50 S-1 and 30 S-1 shear rate,erythrocyte deformation index,erythrocyte accumulation index,erythrocyte rigidity index,and serum IL-1β,IL-8,TNF-α levels of dairy cows with the clinical mastitis were significantly higher than those of the healthy dairy cows（P0.01 or P0.05）.The above indices of dairy cows 7 days after treatment were significantly decreased（P0.01 or P0.05）,however there was no significant difference compared to those of healthy dairy cows（P0.05）.The experiment demonstrated that ＂blood stasis syndrome＂ of the dairy cows with clinical mastitis really existed.Hemorrheologic indexes and serum IL-1β,IL-8,TNF-α could be used for evaluation of therapeutic efficiency in dairy cows with mastitis.The transdermal patch made of compound traditional Chinese medicine had a better curative effect on clinical mastitis,which could improve the microcircul

关 键 词：奶牛 乳房炎 中药 透皮贴剂 血液流变学 细胞因子 

分 类 号：S853.9[农业科学—临床兽医学]