外源腐胺对盐胁迫黄瓜幼苗生长、光合及膜脂过氧化的影响  

Effects of exogenous putrescine concentrations on growth,photosynthesis and lipid peroxidation of cucumber seedlings under salt stress

作　　者：张润花[1,2] 郭世荣[1] 段增强[2] 

机构地区：[1]南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095 [2]中国科学院南京土壤研究所,土壤与农业可持续发展国家重点实验室,江苏南京210008

出　　处：《江苏农业学报》2011年第4期836-841,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家“973”计划项目(2009CB119000);国家自然科学基金项目(30900995、31071831);现代农业产业技术体系建设专项基金项目(Nycytx-35-gw18)

摘　　要：为探明外源腐胺对盐胁迫黄瓜幼苗生长的影响,采用营养液水培法,以耐盐性不同的黄瓜品种长春密刺和津春2号为试材,分析叶面喷施不同浓度腐胺(Putrescine,Put)对NaCl胁迫下黄瓜幼苗株高、鲜重、净光合速率、叶绿素含量、根系活力、MDA含量和电解质渗漏率的影响。结果显示:低浓度(0.1～15.0 mmol/L)Put可缓解NaCl胁迫对两品种黄瓜幼苗生长的抑制,株高、生物积累量、叶片净光合速率显著升高,叶片和根系MDA含量以及电解质渗漏率显著下降,且Put对耐盐品种长春密刺的缓解效果大于盐敏感品种津春2号,两品种幼苗均以10.0mmol/L Put处理的缓解效果最好;高浓度(20.0～30.0 mmol/L)Put处理则加剧了NaCl对两品种黄瓜幼苗的生长胁迫。表明外源Put缓解盐胁迫对黄瓜幼苗的伤害存在浓度效应。The effects of putrescine spraying on plant height,plant fresh weight,photosynthesis rate（Pn）,chlorophyll content,root vigor,MDA content and electrolyte leakage of growing cucumber plants（Cucumis sativus L.salt-tolerant cv.Changchunmici salt-sensitive cv.Jinchun No.2） were investigated using hydroponics with 65 mmol/L NaCl as salt stress.The results were as follows.Salt stress caused the reductions of plant height,plant fresh weight and Pn,but MDA content and electrolyte leakage displayed a tendency to be enhanced.Spraying lower concentrations（0.1-15.0 mmol/L） of putrescine could alleviate the inhibition caused by salt stress,while higher concentrations（20.0-30.0 mmol/L） exhibited the reverse effects.The alleviation effect on Changchunmici was more obvious than on Jinchun No.2.The results on both cultivars showed the best alleviation effect being 10 mmol/L putrescine treatment.These results indicated that there was a concentration effect of putrescine in alleviating damage by salt stress on cucumbers.

关 键 词：腐胺 黄瓜幼苗 盐胁迫 生长 膜脂过氧化 

分 类 号：S642.201[农业科学—蔬菜学]