可吸收性多聚糖止血海绵对创伤愈合的影响  

Effect of absorbable hemostatic polysaccharide sponge on wound healing

作　　者：王青[1] 鲁建国[1] 杨涛[1] 董瑞[1] 杨媛[1] 杨小军[1] 

机构地区：[1]第四军医大学唐都医院普通外科,陕西西安710038

出　　处：《中国普通外科杂志》2011年第8期839-843,共5页China Journal of General Surgery

摘　　要：目的探讨可吸收性多聚糖止血海绵对创伤愈合的影响。方法建立广西巴马小型猪开放创伤模型。横型猪随机分为A(可吸收性多聚糖止血海绵组),B(可吸收性明胶海绵组),C组(空白对照组)。分别于伤后1,3,5,7,14,21,28d观察创面愈合情况;取各时点创面组织行HE染色及免疫组化染色比较各组TGF-β1表达情况、伤后7d创面组织行透射电镜观察。结果伤后5～28d之间各时点,A组创面愈合率均明显高于B组和C组(P<0.05);伤后7d,A组转化生长因子(TGF)-β1在组织中的阳性表达明显高于B组和C组(P<0.05)。透射电镜观察A组以合成分泌旺盛型成纤维细胞为主,B组和C组以增殖型成纤维细胞为主。结论可吸收性多聚糖止血海绵具有促进创伤愈合的作用。Objective To investigate the effect of absorbable hemostatic polysaccharide sponge on wound healing. Methods The open-wound animal models were established in Guangxi Bama minipigs.The porcine models were then randomized into group A（absorbable hemostatic polysaccharide sponge treatment group）,group B（absorbable gelatin sponge treatment group） and group C（no treatment group）.Wound healing responses were monitored at 1,3,5,7,14,21 and 28 d after injury.The histopathological features and transforming growth factor-β 1（TGF-β1） expression of the tissue from wound edge at each time point of each group were examined using HE and immunohistochemical staining and compared,respectively.Furthermore,the ultrastructure of the wound tissues at 7 d after injury was observed by transmission electron microscapy（TEM）. Results The wound healing rate of group A was significantly higher than that of group B and C at the time points from 5 to 28 d after injury（P〈0.05）.The positive expression rate of TGF-β1 in the specimens of group A was significantly higher than that of group B and C,especially at 7 d after injury（P〈0.05）.The results of TEM demonstrated that the fibroblasts in wound tissues of group A were mainly synthetic and secretory type,while they were mainly proliferative type in wound tissues of group B and C. Conclusions Absorbable hemostatic polysaccharide sponge has accelerating effect on wound repair.

关 键 词：伤口愈合/药物作用 可吸收性多聚糖止血海绵 转化生长因子-Β1 

分 类 号：R641.1[医药卫生—外科学]