拟穴青蟹受精膜形成过程中线粒体的变化及功能  

The studies on the mechanism of fertilization membrane formation in Scylla paramamosain mitochondria and its functions

作　　者：陈锦民[1,2] 李少菁[2] 王桂忠[2] 叶海辉[2] 王艺磊[1] 

机构地区：[1]集美大学水产学院,水产科学技术与食品安全省高校重点实验室,福建厦门361021 [2]厦门大学海洋与环境学院,福建厦门361005

出　　处：《水产学报》2011年第8期1139-1145,共7页Journal of Fisheries of China

基　　金：国家“八六三”高技术研究发展计划(2002AA603013);国家自然科学基金项目(31072200);福建省科技厅高校专项基金(2008F5039);福建省高校水产科学技术与食品安全重点实验室开放基金(2008J101);集美大学博士科研启动基金(C60609)

摘　　要：采用透射电子显微镜研究了拟穴青蟹受精膜形成过程中线粒体的变化及其功能。结果显示,大量线粒体首先高度密集于即将发生胞吐的皮质中;随着胞吐的发生,线粒体也随之快速分散到皮层反应部位,线粒体通过分裂、出芽等方式而迅速增多,内质中的线粒体大量向皮质迁移,参与皮层反应;当受精膜开始举起时,线粒体多聚集在新质膜下,参与新质膜的修复和形成;受精膜完全举起后,皮质中的线粒体迅速减少。线粒体在形态、数量及分布等方面的变化,能够反映受精膜形成不同阶段生理活动的旺盛程度以及需能情况,并从细胞水平阐释受精膜形成机制。The changes and functions of mitochondria during the process of fertilization membrane（Fm）formation in Scylla paramamosain were investigated by transmission electron microscope.The results are as follows：Many mitochondria assembled densely at one place in the cortex just before the outburst of the ring-shaped granules.When exocytosis happened,numerous mitochondria scattered rapidly around the cortex where cortical reaction was proceeding.Mitochondria increased dramatically by splitting and budding,meanwhile lots of mitochondria that formed in endoplasm migrated to cortex,as numerous ring-shaped granules involving exocytosis needed abundant energy.While Fm was swelling,most of the mitochondria gathered under the new plasm membrane to participate in its restoring and formation.After Fm swelled completely,mitochondria decreased suddenly and disappeared since cortical granules＇ exocytosis had ceased.In short,the changes of mitochondria on its numbers,shapes and distribution,reflected the trait on physiological activation and energy supply in different stages of Fm formation.From the angle of the changes and functions of mitochondria,this study illuminated the mechanism of Fm formation on cellular level,and provided a new research field for Fm formation in Crustacea.

关 键 词：拟穴青蟹 受精膜 线粒体 皮层反应 

分 类 号：S917.4[农业科学—水产科学]