血管内皮生长因子基因3’-非翻译区936C／T多态性与2型糖尿病外周神经病变相关  

Association between the 936C/T polymorphism in the 3＇-untranslated region of vascular endothelial growth factor gene and diabetic peripheral neuropathy in China

作　　者：邓仰欣[1] 赵远斐[2] 张新焕[1] 

机构地区：[1]泰山医学院附属医院内分泌科,泰安271000 [2]泰山医学院临床医学系2007级

出　　处：《中华内分泌代谢杂志》2011年第8期649-653,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：泰山医学院青年科研基金资助课题

摘　　要：目的探讨血管内皮生长因子（VEGF）基因3’-非翻译区936C／T多态性与山东地区汉族人2型糖尿病合并周围神经病变（DPN）之间的关系。方法194例糖尿病患者分为单纯糖尿病组（n=92）和糖尿病神经病变组（n=102），另120名健康个体设为健康对照组。采用PCR-限制性片段长度多态性（RFLP）方法确定全部个体的基因型；对不同基因型间及病例组间的临床与生化参数、血清VEGF浓度以及VEGF基因936C／T多态性进行了统计分析。结果糖尿病神经病变组C等位基因及CC基因型频率显著高于对照组（Х^2为9．406和9．677，P〈0．05）和糖尿病组（Х^2为5．578和5．614，P〈0．05），而携带T等位基因的基因型（CT＋TT）频率及T等位基因频率显著低于对照组（Х^2为9．406和9．677，P〈0．05）和糖尿病组（Х^2为5．578和5．614，P〈0．05）。Logistic多元回归分析显示血清低密度脂蛋白胆固醇（LDL—C）、总胆固醇、HbA1c水平以及VEGF浓度与DPN发生呈正相关，而VEGF基因936C／T多态性与糖尿病周围神经病变发病危险呈负相关（β=-1．046，OR=0．457，P=0．006，95％CI：0．166—0．741）。结论中国山东地区汉族人群中存在VEGF基因936C／T多态性，C等位基因及CC基因型患者可能是糖尿病易于发生神经病变危险性的遗传标志，而T等位基因和携带T等位基因的基因型（936TF基因型和936CT基因型）可能是降低糖尿病发生神经病变风险的遗传标志。Objective To elucidate the relationship between a 936C/T mutation at 3＇-untranslated region of human vascular endothelial growth factor （VEGF） gene and diabetic peripheral neuropathy （ DPN ）. Methods All subjects recruited in this study were assigned into DM （n = 92, diabetes without neuropathy, retinopathy or nephropathy）, DPN （n = 102, diabetes with peripheral neuropathy only）, and healthy control （n = 120 ） groups, respectively. The gene polymorphism was determined by PCR-RFLP, as well as the other clinical parameters including serum VEGF by ELISA. Results The frequencies of both genotype CC and allele C were significantly higher in DPN group than those in either DM group（Х^2 = 5. 578 and 5. 614, P〈0. 05 ） or control group （Х^2 = 9. 406 and 9. 677, P〈 0.05 ）. However, the frequencies of genotype （CT＋TT） and allele T were significantly lower in DPN group than that in either DM group（Х^2 = 5. 578 and 5. 614, P〈0. 05 ） and control group （Х^2 = 9. 406 and 9. 677, P〈0. 05 ）. The multivariate logistic regression analysis showed that the levels of HbA1c, total cholesterol, low-density lipoprotein- cholesterol（LDL-C） , and serum VEGF positively correlated with DPN, while the 936C/T polymorphism of VEGF gene negatively correlated with DPN （β= -1. 046, OR=0. 457, P=0. 006, 95% CI： 0. 166-0. 741 ）. Conclusions Allele 936C of VEGF gene may serve as a genetic marker susceptible to DPN, while allele 936T may be a protective genetic marker of DPN.

关 键 词：血管内皮生长因子 多态现象 遗传学 糖尿病 2型 糖尿病周围神经病变 

分 类 号：R587.2[医药卫生—内分泌]