阿藿烯对四氯化碳所致大鼠肝纤维化的保护作用  

THE PROTECTIVE EFFECT OF AJOENE ON HEPATIC FIBROSIS INDUCED BY CARBON TETRACHLORIDE IN RATS

作　　者：张晓林[1] 赵树进 杨安平[1] 张相年[2] 翁闪凡[1] 

机构地区：[1]佛山科学技术学院医学院,佛山528000 [2]广州华博生物制药研究所,广州510010

出　　处：《营养学报》2011年第4期393-396,共4页Acta Nutrimenta Sinica

基　　金：广东省自然科学基金项目(No.7010421)

摘　　要：目的探讨阿藿烯对四氯化碳(CCl4)所致大鼠肝纤维化的保护作用。方法将40只SD大鼠随机分为对照组、模型组、低(25mg/kg)和高剂量(50mg/kg)阿藿烯组,用40%CCl4皮下注射制作肝纤维化模型,阿藿烯按低和高剂量分别连续灌胃6w后,检测血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性、总蛋白(TP)和清蛋白(ALB)含量、总胆红素(TBIL)和间接胆红素(DBIL)及肝纤维化的透明质酸(HA)和层粘连蛋白(LN)水平;检测肝组织超氧化物歧化酶(SOD)活性和脂质过氧化(MDA)水平;并进行肝脏组织病理学检查。结果低、高剂量组阿藿烯均可显著降低血清ALT和AST活性、TBIL、DBIL、HA和LN水平(P<0.01或P<0.05);增加ALB含量(P<0.05);提高肝脏SOD活性和减少MDA含量(P<0.01);并明显改善肝脏的纤维化程度(P<0.01或P<0.05)。结论阿藿烯能够减轻CCl4所致的大鼠肝纤维化作用,其机制可能与抗氧化作用有关。Objective To study the protective effect of ajoene on hepatic fibrosis induced by carbon tetrachloride（CCl4）in rats and explore its possible mechanism.Method Forty male Sprague-Dawley rats were divided randomly into four groups： control group,model group,low ajoene group and high ajoene group.The model group was induced by injection of 40% CCl4 2 times per week for 6 w.Low and high ajoene groups were administered with ajoene 25 or 50mg/（kg.d） for 6 w.At the end of the sixth week,serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,total protein（TP）,albumin（Alb）,total bilirubin（TBIL）,direct bilirubin（DBIL）,hyaluronic acid（HA） and laminin（LN） were measured.Hepatic superoxide dismutase（SOD） activity,malondialdehyde（MDA） content and the degree of liver fibrosis were also evaluated.Results The levels of serum ALT,AST,TBIL,DBIL,HA and LN in the ajoene groups were markedly lower than those in the model group（P〈0.01 or P〈0.05）.Ajoene significantly increased SOD activity and reduced MDA content（P〈0.01）.The degree of liver fibrosis were also improved strikingly（P〈0.01 or P〈0.05）.Conclusion Ajoene exhibits inhibitory effect on CCl4-induced hepatic fibrosis in rats by its antioxidation.

关 键 词：阿藿烯 肝纤维化 抗氧化作用 四氯化碳 

分 类 号：R285[医药卫生—中药学]