高速逆流色谱法分离纯化内吗啡肽-1  

Preparative isolation and purification of endormophin-1 by high-speed counter-current chromatography

作　　者：许旭旭[1] 毛政 吴斌[1] 孙洪林[1] 何冰芳[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]浙江省食品药物监督管理局培训中心,浙江杭州310012

出　　处：《化工进展》2011年第9期2064-2068,共5页Chemical Industry and Engineering Progress

基　　金：国家973计划项目(2011CB710800)

摘　　要：利用高速逆流色谱技术对化学酶法合成的内吗啡肽-1粗样进行分离。分析了内吗啡肽-1粗样杂质的分配系数,选择乙酸乙酯-甲醇-水(3∶1.5∶3,体积比)作为两相溶剂系统,上相为固定相,下相为流动相,在主机转速为900 r/min,流速为1.0 mL/min,检测波长为280 nm条件下分离制备。从120 mg粗样中分离得到71 mg样品,内吗啡肽-1的纯度达99.1%,样品回收率为91.5%。该方法简便、快速,为高纯度内吗啡肽-1少量制法提供了技术,并可为高速逆流分离小肽合成产物提供指导。Endormophin-1 synthesized by chem-biological method was purified by high-speed counter-current chromatograph technology（HSCCC）.The optimum separation conditions were as follows：A two-phase solvent system was ethyl acetate-methanol-water（3∶1.5∶3,v/v/v）.The lower phase as the mobile phase was operated at a flow rate of 1.0 mL /min,while the apparatus was rotated at 900 r/min,and detection were length was at 280 nm.Each time 120 mg of the crude sample was loaded under these conditions,and 71 mg of endorphin-1 with the purity of 99.1% was obtained.The recovery was 91.5%.The method was relatively simple,fast,and applicable to the large-scale isolation and separation of endormophin-1.

关 键 词：高速逆流色谱 分离纯化 内吗啡肽-1 

分 类 号：TQ028.98[化学工程]