柴胡中柴胡总皂苷及柴胡皂苷a的含量测定  

Determination of the Content of Saikosaponins and Saikosaponin a in Bupleurum Chinense DC

作　　者：朱兰兰[1,2] 孙玲 翟丽屏 黄伟[1] 李素君[5] 陆永辉[1] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南2500142 [2]天津中医药大学,天津3001933 [3]山东省医药工业研究所,山东济南2501014 [4]山东省地方病研究所,山东济南2500145 [5]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2011年第8期465-467,共3页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金课题(30672649);(81073148);山东省引进国外智力项目(L20083700336);山东省科技攻关关键技术研究课题(2007GG2NS02073);山东省科技平台建设项目(2008GG2NS02021)

摘　　要：目的建立柴胡中柴胡总皂苷及柴胡皂苷a的含量测定方法,并对样品进行含量测定。方法采用紫外分光光度法,以柴胡皂苷a为对照品,在波长545nm处对样品中的总皂苷进行含量测定;采用高效液相色谱法,以C18色谱柱(4.6mm×250mm,5μm)、甲醇-水为流动相,流速为1mL.min-1,检测波长为210nm,测定样品中柴胡皂苷a的含量。结果柴胡总皂苷在30～70μg/mL、柴胡皂苷a在50.4～252μg/mL范围内呈良好的线性关系r,分别为09956、0.9991;平均回收率:柴胡总皂苷为99.31%、柴胡皂苷a为99.22%;RSD:柴胡总皂苷为1.25%,柴胡皂苷a为1.07%。结论本研究所建立的紫外分光光度法测定柴胡总皂苷及高效液相法测定皂苷a含量的方法,简便、易行、快速,结果准确可靠,适用于柴胡中柴胡总皂苷及柴胡皂苷a的含量测定,并为在安全剂量范围内正确使用柴胡提供依据。Objective To establish the method for determination of the content of saikosaponins and saikosaponin a in Bupleurum Chinense DC,and carries on determination of the content of the sample of saikosaponins and saikosaponin a.Methods Saikosaponin a was used as the chemical reference substance determine the content of saikosaponins by UV-Visible Spectrophotometry.The absorbency was measured at 545nm.The content of saikosaponin a in Bupleurum Chinense DC was determined by HPLC.The samples were separated by C18 column（4.6mm×250mm,5μm）.The mobile phase is methyl-alcohol-water（70：30）.The flow is 1mL·min-1.The detector wavelength is 210 nm.Results There was good linearity in the range of 30～70μg/mL（r=0.9956） for saikosaponins、in the range of 50.4～252μg/mL（r=0.9991） for saikosaponin a;The average recovery of saikosaponins and saikosaponin a were 99.31% and 99.22%,and RSD were 1.25% and 1.07%（n=6）.Conclusion The methods for determination of the content of saikosaponins by UV-Visible Spectrophotometry and saikosaponin a by HPLC were proved to be simple,easy and fast to perform,and the results obtained were reliable and suitable.So,it can be used for the quantitative determination of saikosaponins and saikosaponin a in Bupleurum Chinense DC.To provide the basis for correctly using Bupleurum Chinense DC in the safe dose scope.

关 键 词：柴胡总皂苷 柴胡皂苷A 含量测定 

分 类 号：R282[医药卫生—中药学]