8项氧化损伤指标筛检煤工尘肺患者作用比较  

Comparison of 8 oxygenic injury sensitive indexes in screening coal workers' pneumoconiosis

作　　者：范红敏[1] 袁聚祥[1] 秦天榜 邱红燕[1] 王卓[1] 任京雪[1] 

机构地区：[1]河北联合大学公共卫生学院流行病与卫生统计学科(河北省重点实验室-煤炭安全与卫生实验室),河北唐山063000 [2]河北省开滦职业病防治院,063000

出　　处：《中国职业医学》2011年第4期298-301,共4页China Occupational Medicine

基　　金：河北省科技支撑计划项目(09276101D-39)

摘　　要：目的探讨一氧化氮(NO)、丙二醛(MDA)等8项氧化损伤生化指标筛检煤工尘肺(CWP)的价值,为早期发现可疑患者和CWP的防治提供可能的线索。方法分别采用硝酸还原酶法、硫代巴比妥酸显色法、亚硝酸盐形成法、改良Hafenan氏法、尿酸酶-过氧化物酶偶联法、对苯二胺盐酸盐法及化学比色法测定112例CWP患者(CWP组)和144例与CWP组同煤矿的健康接尘对照者(对照组)血浆氧化损伤指标NO、MDA、铜锌超氧化物歧化酶(CuZu-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、尿酸(UA)、铜蓝蛋白(CP)、诱导型一氧化氮合酶(iNOS)及总抗氧化能力(T-AOC)的水平或活力。结果 CWP组与对照组比较,除CP差异无统计学意义(P>0.05)外,其他各项指标比较差异均有统计学意义(P<0.05)。受试者工作特征(ROC)曲线下面积比较,MDA、T-AOC、NO和iNOS的曲线下面积大于基准线的面积,差异有统计学意义(P<0.05)。T-AOC、iNOS、MDA及NO作为CWP的早期筛检指标,以T-AOC最佳,其灵敏度达70.5%,特异度为68.1%。结论 T-AOC是8项CWP早期筛检指标的最佳指标。Objective To study the significance of oxidative injury in the screening of coal workers＇ pneumoconiosis（CWP）.Methods With methods of nitrate reductase and TBA etc,the contents of NO,MDA and other indexes of peroxidation in plasma lipid were detected in 112 patients suffering from CWP and in 114 healthy coal workers.Results There were statistical significances between two groups（P0.05）within the contents or activities of MDA,CuZu-SOD,GSH-Px,T-AOC,UA,NO and iNOS except CP.There were statistical significances in area under the curve（AUC）of MDA,T-AOC,NO and iNOS between the two groups by analyzing ROC.As the screening indexes of pneumoconiosis,MDA,T-AOC,NO and iNOS could be selected as the early screening ones.Especially,T-AOC was the best index of 4 indexes（sensitivity being 70.5% and specificity 68.1%）.Conclusion T-AOC was the best one out of 4 indexes of T-AOC,MDA,iNOS and NO to screen CWP.

关 键 词：煤工尘肺 氧化损伤 筛检 ROC曲线 

分 类 号：R135.2[医药卫生—劳动卫生]