澳洲茄边碱提取纯化工艺及其抗肿瘤作用的研究  

Extraction,purification technology and antineoplastic effects of solamargine

作　　者：唐朝辉[1,2,3] 张岩[1,2,3] 李娜[1,2,3] 徐连明[1,2,3] 赵宾江[1,2,3] 萧伟[1,2,3] 王振中[1,2,3] 毕宇安[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室(筹),江苏连云港222001 [3]江苏省企业院士工作站,江苏连云港222001

出　　处：《中国中药杂志》2011年第16期2192-2195,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-389)

摘　　要：目的:从龙葵中提取纯化澳洲茄边碱,并探讨其抗肿瘤活性。方法:龙葵药材粗粉80%乙醇回流提取,以硅胶柱色谱和重结晶的方法纯化澳洲茄边碱,最后鉴定结构和检查纯度;MTT法筛选澳洲茄边碱对人肿瘤细胞的体外生长抑制作用,并研究其对肝癌H22和EAC小鼠移植瘤的作用。结果:澳洲茄边碱含量达到97.9%;体外对6种肿瘤细胞均具有明显抑制作用,并且在2.4 mg.kg-1的给药剂量下对肝癌H22和EAC小鼠移植瘤具有明显的抑制作用。结论:澳洲茄边碱具有较好的抗肿瘤效果。Objective： To extract and purify of solamargine from Solanum nigrum,and to research its antineoplastic effects.Method： S.nigrum was extracted refluently with 80% alcohol,solamargine was purified with silica gel column chromatography and recrystallization,and then conducted its structure identification and purity checks.Screened the effect on human tumor cell groth inhibition in vitro by MTT assay,and researched on the features in mice with H22 liver cancer or ehrlich ascites tumor of solamargine.Result： The concent of solamargine reached 97.9%.Solamargine had significantly inhibition on 6 tumor cells in vitro,and it had significantly inhibition on mice with H22 liver cancer or ehrlich ascites tumor in the 2.4 mg·kg-1 dose of iv.Conclusion： Solamargine have the antineoplastic effect.

关 键 词：澳洲茄边碱 龙葵 抗肿瘤 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]