HPLC同时测定五味子提取物中的4种类三萜内酯类物质的含量  

Simultaneous determination of 4 nortriterpenoids in Schisandra chinensis extract by HPLC

作　　者：韩晓萍[1,2] 李德坤[3] 周大铮[3] 林瑞超[4] 刘丽芳[1] 叶正良[3] 

机构地区：[1]中国药科大学中药学院,江苏南京211198 [2]青海省药品检验所,青海西宁810003 [3]天津天士力之骄药业有限公司,天津300410 [4]中国药品生物制品检定所,北京100150

出　　处：《中国中药杂志》2011年第16期2226-2229,共4页China Journal of Chinese Materia Medica

基　　金：国家“重大大新药创制”科技重大专项(2010ZX09602-004);重大创新专项(08FDZDSH01404)

摘　　要：目的:研究了HPLC测定五味子提取物中de-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A,lancifodilactone D的含量的方法。方法:Waters Symmetry色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(33∶67),柱温37℃,流速1 mL.min-1,检测波长246 nm。结果:De-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A和lan-cifodilactone D的线性范围和(μg)加样回收率分别为0.075～1.800(98.57%),0.098～0.980(96.44%),0.095～0.950(97.96%),0.053～0.530(97.27%)。结论:该方法分离良好、精密准确、能用于五味子提取物中4种类三萜内酯类物质的定量分析。Objective： To determine 4 nortriterpenoids（de-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A,lancifodilactone D） in Schisandra chinensis extract by HPLC.Method： The analysis was performed on a waters symmetry column（4.6 mm×250 mm,5 μm）with the mobile phase of acetonitrile-water（33∶67） at a flow rate of 1ml· min-1.The column temperature was set at 37℃,and the detector wavelength was 264 nm.Result： The linear ranges of de-hydroxy arisanlactone D,25-hydroxy schindilactone,schindilachone A,and lancifodilactone D are 0.075-1.800,0.098-0.980;0.095-0.950,and 0.053-0.530 μg,respectively,and the average recoveries were 98.57%,96.44%,97.96%,and 97.27%,respectively.Conclusion： The four nortriterpenoids were well separated by this method,and it could be used to determine the four nortriterpenoids in Schisandra chinensis extract.

关 键 词：HPLC 五味子提取物 类三萜内酯 de-hydroxy arisanlactone D 25-hydroxy schindilactone schindilachone A lan-cifodilactone D 

分 类 号：R284[医药卫生—中药学]