小鼠肝癌与肝正常细胞系ST3Gal和ST6Gal家族mRNA表达差异研究  

Different Expression of ST3Gal and ST6Gal mRNA Between Mouse Hepatocellular Carcinoma Cell Lines

作　　者：马汝海[1] 王冬青[2] 潘忠诚[2] 王天骄[2] 何群[2] 赵雨杰[2] 

机构地区：[1]中国医科大学基础医学院化学教研室,中国辽宁沈阳110001 [2]中国医科大学基础医学院教育部细胞生物学重点实验室生物芯片中心,中国辽宁沈阳110001

出　　处：《生命科学研究》2011年第4期323-327,共5页Life Science Research

基　　金：国家自然科学基金资助项目(20672144)

摘　　要：探讨肝癌细胞系Hepa1-6与肝正常细胞系BNL CL.2唾液酸糖基转移酶ST3Gal和ST6Gal家族mRNA表达的差异以及与细胞膜唾液酸含量的关系,采用RT-PCR方法检测ST3Gal唾液酸转移酶家族6个成员以及ST6Gal唾液酸转移酶家族2个成员mRNA表达差异,用凝集素芯片检测细胞膜表面唾液酸表达情况,结果显示:与正常细胞系BNL CL.2相比,hepa1-6细胞内唾液酸转移酶ST3GalⅠ、ST3GalⅣ、ST3GalⅥ呈现高表达,ST3GalⅤ低表达,ST3GalⅡ、ST3GalⅢ表达无显著性差异,两细胞系内均为检测出ST6GalⅠ表达,ST6GalⅡ表达无显著差异;hepa1-6细胞膜α2-3和α2-6连接唾液酸含量均显著增加;提示ST3GalⅠ、ST3GalⅣ、ST3GalⅤ、ST3GalⅥ可能与肝癌发生过程相关,ST3GalⅠ、ST3GalⅣ、ST3GalⅥ可能与肝癌细胞膜α2-3唾液酸含量增加相关,ST6Gal家族对细胞膜α2-6连接唾液酸含量增加无贡献.To investigate the different expression of sialytransferase between liver cancer cell line Hepal-6 and normal cell line BNL CL.2, the mRNAs of ST3Gal family 6 members and ST6Gal family 2 members were detected by RT-PCR. The sialic acid levels on cell membrane surface were analyzed by lectin array. Compared with normal cell line, BNL CL.2, ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅵ were identified highly expressed, ST3Gal V was lowly expressed, ST6Gal Ⅰ was not detected and there was no statistical difference between two cell lines in ST6Gal Ⅱ. The results indicate that ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅴ, ST3Gal Ⅵ may play roles in liver cancer formation and ST3Gal Ⅰ , ST3Gal Ⅳ, ST3Gal Ⅵ are responsible for the increased level of of 2-3 sialic acid on liver cancer cell membrane surface, ST6Gal family members have no correlation with the increased level of a 2-6 sialic acid on liver cancer cell surface.

关 键 词：唾液酸糖基转移酶 肝癌 细胞膜 糖基化 

分 类 号：R392.12[医药卫生—免疫学]