苦杏仁苷对二甲基亚硝胺诱导的大鼠肝纤维化的防治作用  

Prevention effects of amygdalin on rat fibrosis induced by dimethylnitrosamine

作　　者：李雪梅[1,2] 冯琴[1] 彭景华[1] 傅琪琳[1] 唐亚军[1] 赵瑜[1] 胡义扬[1] 

机构地区：[1]上海中医药大学附属曙光医院,上海中医药大学肝病研究所,肝肾疾病病证教育部重点研究室,上海市中医临床重点实验室,上海市高校中医内科学E-研究院 [2]天津中医药大学第一附属医院

出　　处：《中西医结合肝病杂志》2011年第4期221-223,I0002,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然基金面上项目(No.30973924);教育部博士点基金(No.2007268002);上海市教委科研创新项目(No.08ZZ64);上海市教委创新团队(第1期);国家中医药管理局中医肝胆病重点学科项目

摘　　要：目的:探讨苦杏仁苷对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的防治作用。方法:采用Wistar雄性大鼠,以腹腔注射DMN诱导大鼠肝纤维化模型。在造模4周后,将造模大鼠按体重分层随机分为模型组、苦杏仁苷组及对照药秋水仙碱组,每组10只,灌胃用药两周后取材。观察检测以下指标:①末次体重、肝脾比值;②肝组织羟脯氨酸含量;③肝功能指标:血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、谷胺酰转肽酶(GGT)活性;白蛋白(Alb)、总胆红素(TBil)含量;④肝组织天狼星红染色及HE染色;⑤肝组织脂质过氧化指标:超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)。结果:①模型组大鼠肝组织羟脯氨酸含量与正常组相比显著升高(P<0.01);与模型组相比,苦杏仁苷及秋水仙碱治疗组大鼠肝组织羟脯氨酸含量显著降低(P<0.01);②天狼星红染色显示模型组大鼠窦周胶原沉积明显,肝小叶间形成较厚汇管区和中央静脉间的纤维间隔,可见较多完整的假小叶,HE染色结果显示模型组大鼠肝细胞水肿明显,汇管区结缔组织有不同程度增生,肝组织中有炎性细胞浸润,窦壁细胞增生明显;苦杏仁苷治疗组和秋水仙碱对照组与模型组相比炎症缓解、胶原纤维增生程度分期显著降低(P<0.05);③模型组大鼠血清ALT、AST、GGT活性及TBil含量显著升高,Alb含量显著降低(P<0.01);苦杏仁苷组与秋水仙碱组大鼠血清ALT、AST活性显著降低(P<0.01或P<0.05);④与正常组比较,模型组大鼠肝组织SOD活性、GSH含量及GSH-Px活性显著降低,MDA含量显著升高(P<0.05);苦杏仁苷能显著升高模型大鼠肝组织GSH活性(P<0.05),秋水仙碱则能显著升高GSH、GSH-Px活性。结论:苦杏仁苷对DMN诱导的大鼠肝纤维化有显著改善作用。Objective：To study the prevention effects of amygdalin on rat fibrosis induced by DMN.Methods：Fibrosis model in rats was duplicated with DMN.After being injected with DMN for 4 weeks,model rats were randomly divided into model group,amygdalin treated group and colchicine treated group.Samples were harvested at the end of 6th week after being given corresponding drugs for 2 weeks.The followings were detected： ①The content of liver Hydroxyproline（Hyp）;②The liver pathology（Sirius red staining and HE staining）;③The activity of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,γ-glutamyltransferase（γ-GT） and the content of albumin（Alb）,total bilirubin（TBil） in serum;④The activity of liver superoxide dismutase（SOD）,Glutathione（GSH）,glutathione peroxidase（GSH-Px）and the content of liver maleic dialdehyde（MDA）.Results：①The content of liver Hyp increased significantly in model group,but it significantly decreased in amygdalin treated group and colchicine treated group;②There were serious fibrosis and inflammation in model group.Amygdalin and colchicine can obviously alleviate fibrosis and inflammation;③Activity of serum ALT,AST,γ-GT and content of serum TBil of the model group significantly increased compared with the normal group;ATL and AST decreased significantly in amygdalin treated group and colchicine treated group;④There were significant increases in MDA and significantly decrease in SOD,GSH and GSH-Px.Amygdalin could obviously increase GSH.Colchicine could obviously increase GSH and GSH-Px.Conclusion： Amygdalin has good experimental prevention efficacy on rat fibrosis induced by DMN.

关 键 词：肝纤维化 苦杏仁苷 二甲基亚硝胺 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]