肝泰胶囊对大鼠酒精性肝病的防治作用  

Effect of Gantai capsule on alcohol liver disease in rats

作　　者：曹芹芳[1] 虞涤霞 王密[1] 吴翠环[3] 舒柏华[4] 李安意[5] 唐望先[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝病研究所,湖北武汉430030 [2]武汉市七医院 [3]华中科技大学同济医学院病理科 [4]华中科技大学同济医学院公共卫生学院 [5]华中科技大学同济医学院附属同济医院动物试验研究中心

出　　处：《中西医结合肝病杂志》2011年第4期231-234,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨肝泰胶囊对大鼠酒精性肝病(ALD)的防治作用及其可能的机制。方法:将40只SD雄性大鼠随机分为正常组10只,采用普通饲料喂养;模型组18只,高脂饲料喂养,同时灌喂酒精性混合液;预防组12只,高脂饲料喂养,同时给予肝泰胶囊和酒精混合液。实验时间为4个月。采用光镜、电镜观察大鼠肝组织病理学改变;M asson染色观察各组大鼠肝纤维化程度;采用放射免疫法测定血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ-)、Ⅳ型胶原(Ⅳ)的含量;用半自动生化仪检测丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST);分光光度仪检测丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、γ-谷氨酰转肽酶(γ-GT);免疫组化检测α-平滑肌蛋白(α-SMA)抗体的表达。结果:预防组与模型组比:①预防组大鼠肝组织肝纤维化程度明显改善;②肝组织胶原纤维面积明显减少;③血清透明质酸显著降低;④肝功能损伤明显减轻。结论:肝泰胶囊能显著改善酒精性肝病大鼠的肝损伤,提示该药对酒精性肝病有一定的防治作用。Objective：To investigate the possible mechanism of Gantai capsule on resisting hepatic fibrosis induced by alcohol in rats.Methods： Forty rats were randomly divided into control group,model group and Gantai-treated prophylaxis group.Light and electron microscope were adopted to observe the histopathology of the hepatic tissue.Masson dyeing and PC-Ⅲ,HA,Ⅳ-C in the serum were evaluated to reflect the level of hepatic fibrosis.Meanwhile,blood,lipids,liver MDA,SOD,and GSH were measured and then used immunohistochemistry to measure the expression of α-SMA in liver tissue.Results：Compared with model group,it revealed that Gantai-treated prophylaxis group rats： ① The degree of fibrosis was improved.② Liver collagen fibers markedly was decreased.③ Serum hyaluronic acid and laminin obviously was decreased.④ Liver function was improved.Conclusion：Gantai capsule has an effect on alcohol liver disease and can significantly alleviate the alcohol-induced hepatic fibrosis injury in rats.

关 键 词：肝泰胶囊 酒精性肝病 大鼠 

分 类 号：R285.5[医药卫生—中药学]