RP-HPLC法测定男宝胶囊中补骨脂素和异补骨脂素含量  

Determination of psoralen and isopsoralen in Nanbao capsules by RP-HPLC

作　　者：宋剑锋 张志根 张成川 

机构地区：[1]浙江衢州市食品药品检验所,衢州324002 [2]浙江乐清市中医院,温州325000

出　　处：《海峡药学》2011年第8期73-75,共3页Strait Pharmaceutical Journal

摘　　要：目的建立一种可测定男宝胶囊中补骨脂素和异补骨脂素的RP-HPLC方法。方法采用Shim-pack VP-ODS(250mm×4.6mm 5μm)为色谱柱;流动相为甲醇-水(45∶55);检测波长为246nm;流速为1.0mL.min-1;柱温为40℃;峰面积外标法定量;结果本法补骨脂素在9.10×10-4μg^0.2742μg范围内呈良好的线性关系,(r=0.999 99),平均回收率为99.0%,RSD 1.57%;异补骨脂素在9.90×10-4μg^0.2970μg范围内呈良好的线性关系,(r=0.999 99),平均回收率为100.7%,RSD 1.76%。结论本方法简便、快速、准确可靠,可作为该制剂的质控方法。OBJEICTIVE To establish a RP-HPLC method for determining the content of Psoralen and isopsoralen in Nanbao Capsules.METHODS A Shim-pack C18 column（250mm×4.6mm 5μm）was used.The mobile phase was methanol-water（45∶55）.The detection wavelength was 246nm.RESULTS The linear range of paoralen was 9.10×10-4μg^0.2742μg（r=0.999 99）.The average recovery（n=6） was 99.0%.RSD was 1.57%.The linear range of isopaoralen was 9.90×10-4μg^0.2970μg（r=0.99999）.The average recovery（n=6） was 100.7%.RSD was 1.76%.CONCLUSION The method is simple,reliable,accurate and can be applied to the quality control of the preparetion

关 键 词：男宝胶囊 补骨脂素 异补骨脂素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]