优选复方黄芩温敏凝胶的壳聚糖絮凝法澄清工艺  

Optimize the clarification process of compound Huangqin temperature sensitive gel with chitosah

作　　者：潘旭东[1] 林雄[1] 黄燕[1] 程心玲[1] 陈肖虹[1] 

机构地区：[1]福建中医药大学附属人民医院,福州350004

出　　处：《海峡药学》2011年第8期87-89,共3页Strait Pharmaceutical Journal

基　　金：福建省教育厅课题项目(项目编号:JB09146)

摘　　要：目的优选壳聚糖对复方黄芩温敏凝胶的最佳澄清工艺。方法采用HPLC法,以黄芩苷的含量为评价指标,以正交试验法L9(34)优选澄清工艺参数。结果最佳澄清工艺为壳聚糖加入量0.4mL.g-1,药液静置时间24h,药液稀释比为1∶6。结论此工艺对黄芩苷保留率高,稳定性好,成本低,可用于复方黄芩温敏凝胶的精制。OBJECTIVE To optimize the clarification process of compound Huangqin temperature sensitive gel with chitosan.METHODS With the content of Baicalin as index,using HPLC,the clarification process was optimized by orthogonal design L9（34）.RESULTS The best clarification process was that the amount of chitosan was 0.4 mL·g-1,holding time was 24h,and dilution ratio was 1∶6.CONCLUSION The process is stable,and good for retention rate of Baicalin,and it has low cost and be suitable for the purification of compound Huangqin temperature sensitive gel.

关 键 词：黄芩苷 正交试验 HPLC 壳聚糖 

分 类 号：TQ460.4[化学工程—制药化工]