α-硫辛酸对大豆农杆菌介导GUS瞬时表达和芽诱导的影响  

Effect of α-Lipoic Acid on the Agrobacterium-tumefaciens Mediated GUS Transient Expression Rate and Shoot Induction Percentage in Soybean(Glycine max L.)

作　　者：杨晓凤[1] 卢涛[1] 周正剑[2] 寿惠霞[2] 唐桂香[1] 

机构地区：[1]浙江大学农业与生物技术学院作物科学研究所,浙江杭州310058 [2]浙江大学生命科学学院植物科学研究所,浙江杭州310058

出　　处：《大豆科学》2011年第4期552-556,共5页Soybean Science

基　　金：国家转基因专项资助项目(2009ZX08010-013B;2008ZX08004-0004;2008ZX08004-001);国家自然科学基金资助项目(31071443)

摘　　要：以适宜转化大豆受体YC-2为材料,研究了抗氧化剂α-硫辛酸对农杆菌介导GUS瞬时表达和芽诱导率的影响。结果表明:在共培养基中添加不同浓度的抗氧化剂可显著降低外植体的褐化率,随α-硫辛酸浓度的增加外植体褐化率明显减少,但白化率增加,且这些白化的外植体在后期不易产生诱导芽;共培养中添加不同浓度的α-硫辛酸可增强GUS瞬时表达率和芽诱导的再生率,且以25 mg·L-1效果最佳。Antioxidant-α-lipoic acid（LA）influencing on the Agrobacterium-tumefaciens mediated GUS transient expression rate and shoot induction percentage in soybean cultivar＇YC-2＇was studied in this paper.The results showed the explant browning percentage was markedly decreased after added different concentrations of α-lipoic acid in the co-cultivation medium.In the meantime,the explant whitening percentage was increased with the increased concentration of α-lipoic acid in co-cultivation medium.Meanwhile,the whitening explant couldn＇t induce shoot regeneration after culured on the shoot induction medium for a month.It also showed the transient GUS expression rate and shoot induction frequency were also increased after added different LA concentrations in the co-cultivation medium.The optimized LA concentration was 25 mg·L-1in the co-cultivation medium.

关 键 词：大豆 Α-硫辛酸 GUS瞬时表达 芽诱导率 

分 类 号：S565.1[农业科学—作物学]