芽孢杆菌对大豆根腐病防治效果研究  

Control Efficacy of Bacillus spp.to Soybean Root Rot

作　　者：朱文静[1] 伍辉军[1] 高学文[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京210095

出　　处：《大豆科学》2011年第4期621-625,共5页Soybean Science

基　　金：国际科技合作资助项目(2009DFA32740);公益性行业(农业)科研专项资助项目(3-23);国家高技术研究发展计划(863计划)资助项目(2006AA10Z172);国家大学生创新性实验计划资助项目(091030707)

摘　　要：选择20株从西藏地区植物根际土壤分离鉴定的具有生防潜力的芽孢杆菌,通过平板抑菌试验,筛选出对大豆根腐病病原菌尖孢镰刀菌嗜管专化型(Fusarium oxysporumf.sp.tracheiphilum)拮抗效果好的菌株11株,并利用这些菌株的发酵液作为活性成分,添加一定比例的各种助剂,制备出生物种衣剂。室内沙培试验结果表明:由萎缩芽孢杆菌LSSC3和枯草芽孢杆菌SYST2制备的种衣剂对大豆发芽及生长都有显著促进作用。用这2种生物种衣剂对大豆进行温室盆栽试验,在接种有病原菌的条件下,由2种生物种衣剂包衣大豆的株高、鲜重、主根长等都显著的高于化学种衣剂包衣和未包衣处理,同时对大豆根腐病也起到了很好的防治作用,防效分别达72.38%和73.69%。Twenty Bacillus strains,with biocontrol potential,were selected from many Bacillus strains which were isolated from rhizosphere soil of plants in Tibet.Through the experiment of dual-culture test in plate,we selected 11 strains which showed strong inhibitory to Fusarium oxysporum f.sp.tracheiphilum,and prepared biological seed coating agent by using their fermentation liquor as active ingredient,and a certain proportion of different material as additives.The indoor test demonstrated that seed coating agent which were prepared with LSSC3 and SYST2 strains could enhance the germination and growth of soybean.The results of greenhouse experiment which had inoculated with pathogenetic fungi in soil showed that,the plant height,fresh weight and root length of soybeans which treated with these two biological agents were evidently higher than chemical agent and uncoated treatment.Besides,two biological seed coating agents could control soybean root rot disease effectively,with good control efficiency of 72.38% and 73.69%.

关 键 词：芽孢杆菌 生物种衣剂 大豆 促生 生防 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]