野生大豆苯丙氨酸解氨酶(PAL)在不同诱导条件下变化规律  

Response of Phenylalanine Ammonia-lyase(PAL)to Different Inducing Conditions in Wild Soybean(Glycine.soja)

作　　者：张必弦[1,2] 胡小梅[1] 朱延明[1] 来永才[2,3] 李炜[3] 肖佳磊[3] 李琬[3] 毕影东[3] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院博士后工作站,黑龙江哈尔滨150086 [3]黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2011年第4期703-705,709,共4页Soybean Science

基　　金：国家科技支撑计划资助项目(2006AB13B05);农业部转基因新品种培育科技重大专项资助项目(2008ZX08009-003);黑龙江省青年科学基金资助项目(QC2010053)

摘　　要：以野生大豆(龙野01-177)为材料,根据PAL酶活性的影响因素,选取48 h为一个周期,每3 h进行取样,研究低温(-4℃)诱导、机械损伤诱导、紫外线照射诱导、激素BAP诱导和正常对照组条件下的酶活性、蛋白含量、酶比活性的变化。结果表明:4种诱导条件下野生大豆PAL的酶活性和蛋白质含量变化趋势均相同且表现出不同的变化规律;通过比较酶比活性,发现4种诱导条件中,低温诱导效果最好。Wild Soybean（Glycine soja） with more desired traits is the ancestor of the cultivated soybean（Glycine max）,and phenylalanine ammonia-lyase（PAL）is the key enzyme in the synthesis of isoflavones.With wild soybean（longye 01-177）as material,according to the influencing factors of PAL activities,selecting each 48 h for a cycle and sampling for each 3 h,we studied the enzyme activity,protein content and the variation of enzyme activity under the conditions of low temperature（-4℃）（LT）,mechanical damage（MD）,ultraviolet ray（UV）,hormone（BAP）and normal controls conditions.The results indicated that there was the same change trend of the enzyme activity and protein content of wild soybean PAL under different induction conditions and low temperature had the best induced effect.

关 键 词：野生大豆 苯丙氨酸解氨酶 诱导条件 

分 类 号：S565.1[农业科学—作物学]