原因不明复发性流产患者蜕膜组织中Th17/Treg免疫失衡研究  

The Th17/Treg imbalance in deciduas of unexplained recurrent spontaneous abortion

作　　者：秦卫兵[1] 杨宁[1] 韦成厚[1] 何小红[1] 崔蓉[1] 苗竹林[1] 许玉芳[1] 

机构地区：[1]广东省计划生育科学技术研究所,广州510600

出　　处：《广东医学》2011年第15期1956-1959,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30901610);广东省科技计划项目(编号:2008B030301193)

摘　　要：目的探讨原因不明复发性流产(URSA)患者蜕膜组织中Th17/Treg免疫平衡情况。方法应用流式细胞技术检测URSA患者和正常早孕者蜕膜组织中Th17和Treg细胞频率,应用real-time PCR方法检测转录因子RORc和Foxp3 mRNA水平。结果 URSA患者蜕膜组织中Th17细胞在淋巴细胞和CD4+T细胞中的频率显著高于正常早孕者(P<0.001),URSA患者蜕膜组织中Treg细胞在淋巴细胞和CD4+T细胞中的频率显著低于正常早孕者(P<0.001)。URSA患者蜕膜组织中RORc mRNA水平明显高于正常早孕者,而Foxp3 mRNA水平显著低于正常早孕者(P<0.001)。结论 URSA患者蜕膜组织中存在明显的Th17/Treg免疫失衡,可能是URSA发生的重要原因。Objective To investigate the imbalance Thl7/Treg in deciduas of unexplained recurrent spontaneous abortion （URSA）. Methods In URSA patients and health early pregnant subjects, Thl7 and Treg cells frequencies were measured by flow cytometry; while RORc and Foxp3 mRNA levels were measured by real - time PCR. Results There was significant increase in decidual Thl7 cell frequency and RORc mRNA level in URSA patients （P 〈 0. 001 ）. Meanwhile, there was significant reduction in decidual Treg cell frequency and Foxp3 mRNA level in URSA patients （ P 〈 0. 001 ）. Conclusion The decidual Thl7/Treg imbalance plays an important role in URSA.

关 键 词：原因不明复发性流产 TH17细胞 TREG细胞 

分 类 号：R714.21[医药卫生—妇产科学]