人BAFF-R-IgG4mut融合蛋白对BXSB狼疮鼠CD4^+T细胞增殖活性的影响  

The effect of BAFF-R-IgG4mut fusion protein on proliferative activity of CD4^+T lymphocytes in murine BXSB lupus

作　　者：丁涵露[1] 王莉[1] 倪兵[2] 高闻达[3] 钟雪梅[4] 

机构地区：[1]四川省人民医院肾内科,四川成都610072 [2]第三军医大学免疫学研究所,重庆400038 [3]哈佛大学Beth Israel Deaconess医学中心免疫研究所 [4]波士顿大学医学中心

出　　处：《四川医学》2011年第8期1157-1160,共4页Sichuan Medical Journal

基　　金：四川省卫生厅课题资助项目(编号:303005002239032)

摘　　要：目的观察人BAFF-R-IgG4mut融合蛋白对BXSB狼疮鼠CD4+T淋巴细胞增殖活性的影响。方法体外分离、纯化BXSB狼疮鼠脾脏CD4+T细胞,流式细胞技术检测CD4+T细胞BAFF-R表达,MTT法观察不同剂量BAFF-R-IgG4mut融合蛋白对BAFF刺激的CD4+T细胞增殖活性的影响。结果台盼蓝染色分离后的BXSB狼疮鼠脾脏CD4+T细胞活性率为(93.6±3.2)%;流式细胞技术检测BXSB狼疮鼠脾脏CD4+T细胞表达BAFF-R;100ng/ml、200ng/ml、400ng/ml BAFF-R-IgG4mut蛋白作用于BAFF和CD3抗体刺激的CD4+T细胞后,CD4+T细胞增殖抑制率分别是56.24%、67.07%和75.45%,较单纯BAFF+CD3抗体组差异有统计学意义(P<0.05)。结论 BAFF-R-IgG4mut融合蛋白可体外抑制BXSB狼疮鼠CD4+T细胞增殖活性。Objective Study the effect of human BAFF-R-IgG4mut fusion protein on proliferative activity of CD4＋T lymphocytes in murine BXSB lupus.Methods Isolation and purification of BXSB lupus mouse spleen CD4＋T cells.BAFF-R expression in CD4＋T cells was detected by flow cytometry.The effect of different doses of BAFF-R-IgG4mut fusion protein on the proliferative activity of CD4＋T cell under BAFF and CD3 antibody stimulation were assessed by MTT.Results Activity of CD4＋T cells separatiing from BXSB lupus mouse were（93.6±3.2）% through detection by Trypan blue staining.Spleen CD4＋T cells in BXSB lupus mice could express BAFF-R.After 100ng/ml,200ng/ml,400ng/ml BAFF-R-IgG4mut protein stimulating the CD4＋T cells,the CD4＋T cell prolliferative inhibition rate were 56.24%,67.07% and 75.45% respectively which were significant different than that of BAFF＋CD3 antibody group（P0.05）.Conclusions BAFF-R-IgG4mut fusion protein inhibited proliferative activity of CD4＋T cell in BXSB lupus mice in vitro.

关 键 词：B细胞刺激因子受体 B细胞刺激因子 BAFF-R-IgG4mut融合蛋白 CD4+T淋巴细胞 

分 类 号：R593.242[医药卫生—内科学]