沙利度胺干预氧化乐果中毒致SD大鼠肺损伤的实验研究  

Experiment on thalidomide intervention in rats′lung injury induced by omethoate poisoning

作　　者：李孟秦[1] 曹小平[1] 

机构地区：[1]川北医学院附属医院急诊医学科,四川南充637000

出　　处：《四川医学》2011年第8期1181-1183,共3页Sichuan Medical Journal

摘　　要：目的通过测定氧化乐果中毒SD大鼠血清TNF-α浓度、肺组织TNF-α表达、肺系数,探讨沙利度胺干预氧化乐果中毒致SD大鼠肺损伤的治疗价值。方法①150只健康雄性SD大鼠随机分为3组:空白对照组(A组)、染毒组(B组)、沙利度胺预处理组(C组),A组30只,B组、C组各60只。②B、C组大鼠氧化乐果灌胃染毒(45mg/kg),C组在染毒前22h及2h沙利度胺50mg/kg灌胃预处理,A组大鼠等容等剂量生理盐水灌胃作为空白对照。③染毒或生理盐水灌胃后30min、3h、6h、12h、24h、48h处死大鼠,测定各组肺系数、血清TNF-α浓度、肺组织TNF-α表达水平,并观察肺组织病理改变。结果①大鼠染毒后B、C组血清TNF-α浓度、肺系数增高以及肺组织TNF-α表达上调(P<0.01),C组与B组相比大鼠血清TNF-α浓度、肺系数减低以及肺组织TNF-α表达下调(P<0.01);②B、C组肺组织于染毒后30min～6h出现逐渐加重的肺间质充血、肺泡间隔增宽,局部可见肺泡内红细胞聚集,12～48h逐渐减轻。结论氧化乐果中毒致SD大鼠TNF-α水平增高,可能是导致肺损伤的重要因素之一,沙利度胺灌胃预处理能有效干预氧化乐果中毒致SD大鼠肺损伤,为临床治疗提供了新的思路。Objective To explore the therapeutic value of thalidomide intervention in the treatment of rats＇ lung injury induced by omethoate poisoning by observing the changes of the blood serum TNF-α concentrations,TNF-α level of lung tissue and lung coefficient.Methods ①150 healthy male SD rats were randomly divided into three groups：group A（control,30 rats）,group B（omethoate gavage exposure,60 rats）,group C（administer thalidomide before omethoate gavage exposure,60 rats）.②Rats in group A and group B were treated omethoate by gavage（45mg/kg）.Thalidomide（50 mg/kg） was administered orally 22 hours and 2 hours before omethoate gavage exposure in group C.Rats in group A were treated normal saline as a blank control.③Then kill the rats at designated observing point（30min、3h、6h、12h、24h、48h after be treated with omethoate or normal saline）,determine the lung coefficient,blood serum TNF-α concentration and TNF-α level of lung tissue.At the same time,pathological changes of lung tissue were also observed.Results ①There was significant difference（P0.01） among the three groups in blood serum TNF-α concentration,TNF-α level of lung tissue and the level of lung coefficient,with Group B the highest,followed by Group C,and Group A,the lowest.②Lungs of group B and C showed pathological changes from 30 minutes to 6 hours after omethoate gavage,with lung stroma engorged,alveolar septum widened and some alveoli pulmonis full of red blood cells.The pathological changes gradually aggravated from 30 minutes to 6 hours and gradually mitigated from 12 hours to 48 hours after omethoate gavage.Conclusion The increasing TNF-α level may be one of the important factors leading to lung injury induced by omethoate poisoning.Thalidomide pretreatment can reduce the degree of lung injury in the rats poisoned by omethoate,which may be a new direction for clinical treatment.

关 键 词：氧化乐果 肺损伤 TNF-Α 沙利度胺 

分 类 号：R965[医药卫生—药理学]