中华乌塘鳢雌雄异体和雌雄同体性类固醇激素含量的季节变化与差异  

Seasonal changes and differences of concentrations of sex steroid hormones in gonochoristic and hermaphroditic Bostrichthys sinensis

作　　者：李志杰[1] 洪万树[1] 连惠波[1] 赖晓健[1] 王琼[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005

出　　处：《台湾海峡》2011年第3期369-374,共6页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金资助项目(40776080);福建省自然科学基金资助项目(2007J0068)

摘　　要：采用酶联免疫吸附法测定了野生中华乌塘鳢(Bostrichthys sinensis)不同季节血清E2和11-KT含量的变化,并比较了生殖季节养殖雌雄同体与野生雌雄异体之间E2、11-KT和17α,20β-P三种性类固醇激素含量的差异.结果表明,野生雌鱼和雄鱼血清E2和11-KT的含量均具有明显的季节变化,其高低均依次为5>8>2>11月.在相同月份,雌鱼的E2含量显著高于雄鱼,而雄鱼的11-KT含量显著高于雌鱼.生殖高峰期,雌雄同体E2含量高于雌雄异体雄鱼,但低于雌雄异体雌鱼;雌雄同体11-KT和17α,20β-P含量均高于雌雄异体雌鱼和雄鱼;野生雌鱼和雄鱼血清17α,20β-P含量没有显著差异.性类固醇激素在中华乌塘鳢的生殖过程中起着重要作用,E2是野生中华乌塘鳢雌鱼的主要雌激素,而11-KT则是野生雄鱼的主要雄激素.The changes in the levels of serum 17~estradiol （E2） and 11-keto-testosterone （I1-KT） of wild Bostrichthys sinensis during different seasons were measured using enzyme-linked immunosorbent assay （ELISA）.Differences in levels of three sex steroid hormones, E2, ll-KT, and 17,2013-dihydroxypregn-4-en-3-one （ 17α, 20β-P） were compared between hermaphroditic and gonochoristic Chinese black sleepers during the breeding seasons. The study found that the levels of serum E2 and 11-KT correlated with seasonal changes in both wild females and males. The levels of E2and 11-KT peaked in May, declined sequentially in August, February, and November. In the same months, the levels of serum E2 in females （ 165.05 ± 18.00 pg/cm3 in February, 1 461.7 ±313.95 pg/em3 in May, 640.58 ＋ 35.34 pg/em3 in August, and 132.62 ＋ 21.57 pg/em3 in November） were significantly higher than those in males （40.87 ＋8.16 pg/cm3in February,70.33 ± 11.08 pg/cm3 in May, 56.44 ±8.46 pg/cm3 in August, and 27.59 ＋7.66 pg/em3 in November）. The levels of ll-KT in males （40.37 ＋8.44 pg/em3 in Feb- ruary, 318.89 ＋ 15.02 pg/em3 in May, 44.12 ＋2. O1 pg./em3 in August, and 31.41 ＋2.87 pg/cm3 in November） were significantly higher than those in females （10.49 ＋2.87 pg/em3 in February, 55.99 _＋ 8.8 pg/cm3 in May, 27.65 ＋ I. 47 pg/em3 in August and 9.18±2.39 pg/cm3 in November）. The levels （398.02 ±248.19 pg/em3 ） of serum E2 in the hermaphroditic Chinese black sleepers were higher than those in wild males （294.65 ± 16.26 pg/em3） , but lower than those in wild females （ 1 461.7 ± 313.95 pg/cm3 ） during the peak spawning seasons. The levels of serum 11-KT and 17or, 2013-P in the hermaphroditic Chinese black sleepers were both higher than those in the gonoehoristie females and males. There were no significant differences between the levels of serum 17ot,2013-P of males and females. It can be inferred from this study that sex steroid hormones play important roles in the reproduction of Chinese b

关 键 词：海洋生物学 中华乌塘鳢 雌雄异体 雌雄同体 性类固醇激素 

分 类 号：P735[天文地球—海洋生物学]