海洋放线菌ACMA006抗肿瘤活性成分的分离纯化及结构鉴定  

Separation,purification,and structural identification of antitumor active components from marine actinomycete ACMA006

作　　者：曹雪[1] 杨瑞丽[1] 袁献温[1] 奚涛[2] 杨臻峥[2] 

机构地区：[1]东南大学医学院病原生物学和免疫学系,江苏南京210009 [2]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《台湾海峡》2011年第3期400-404,共5页Journal of Oceanography In Taiwan Strait

基　　金：江苏省卫生厅科研基金资助项目(H200758)

摘　　要：对从中国江苏连云港海域中筛选出的海洋放线菌ACMA006的发酵产物进行了分离纯化,获得2种抗肿瘤活性化合物,并进一步鉴定其结构.海洋放线菌ACMA006经大规模发酵后,发酵液采用溶剂萃取法提取其中的抗肿瘤活性成分,并利用硅胶柱层析、制备薄层及HPLC等方法对萃取物进行分离得到单体化合物.利用红外光谱、质谱、核磁共振1H-HMR谱和13C-NMR谱等波谱数据对单体化合物的结构进行解析和鉴定.结果表明化合物B为放线菌素D,其分子式为C62H86N12O16,含有2个多肽酯环,由L-苏氨酸、D-缬氨酸、L-脯氨酸、N-甲基甘氨酸和L-N-甲基缬氨酸组成,通过羧基与发色母核吩噁嗪酮相连接.化合物A可能为放线菌素D的衍生物.这是国内外首次从海洋放线菌中分离提取出放线菌素D.Actinomycete ACMA006 was obtained from the sea near Lianyungang, Jiangsu Province in eastern China and showed anti-tumor activities. Two active antitumor compounds were obtained through large-quantity fermentation and purification of the marine actinomycete ACMA006. The structures of monomeric compounds were further analyzed and identified through spectral analyses that included IR, MS, NMR, I H-HMR, 13C-NMR, among others. Based on spectral data, compound B was identified as actinomycin D and its molecular formula was C62Hs6Nj2OI6, containing two cyclic pentapeptides composed of L-Thr, D-Val, L-Pro, N-methyl glycoeol, and LN-methylvaline linked with phenoxazone chromophore via carboxyl group. Compound A was suspected to be a derivative of actinomycin D. To our knowledge, this study is the first description of the extraction of actinomycin D from a marine actinomycete.

关 键 词：海洋生物学 放线菌 抗肿瘤活性 放线菌素D 分离纯化 结构鉴定 

分 类 号：Q178.53[生物学—水生生物学]