澳洲淡水鳄线粒体基因组全序列的测定及结构分析  

The Complete Mitochondrial Genomes of Crocodylus Johnstoni

作　　者：胡月红[1] 张曼[1] 王亦舒[1] 晏鹏[1] 吴孝兵[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2011年第4期353-358,共6页Journal of Anhui Normal University(Natural Science)

基　　金：国家自然科学基金资助(30470213)

摘　　要：运用PCR和分子克隆的技术,获得了澳洲淡水鳄(Crocodylus johnstoni)的线粒体基因组全序列(mtDNA).其序列全长为16,857bp,由22个tRNA、2个rRNA和13个蛋白编码基因及非编码的控制区(control region)组成,碱基组成为:31.99%A,28.82%C,14.90%G,24.29%T.同其它鳄类一样,澳洲淡水鳄发生了基因重排,即tRNAPhe和tRNASer(AGY)的基因重排现象.虽然与典型的脊椎动物线粒体基因排列顺序不同,但在已测出的所有鳄类中,各基因的排序是一致的,显示了鳄类mtDNA的高度保守性.The complete mitochondrial DNA（mtDNA） molecule of the Australian freshwater crocodile（Crocodylus johnstoni）was obtained by Polymerase Chain Reaction （PCR） and molecular cloning.The complete mitochondrial genome of this crocodilian was 16,857 bp in length.The organization conformed to that of other vertebrate mtDNA,including 22 tRNAs,two rRNAs,13 protein-coding genes,and a control region.The base composition was 31.99% A,24.29% T,28.82% C,14.90% G.The gene arrangement of the genome was the same as that of published crocodilians,and we can found two tRNA genes differ from other vertebrates,showing that the gene order of crocodilians is remarkably conserved.

关 键 词：澳洲淡水鳄(Crocodylus johnstoni) 线粒体全基因组 基因组结构 

分 类 号：Q959.3[生物学—动物学]