麦冬皂苷D的分离提纯  

Separation and purification of ophiopogonin D

作　　者：邓惠方[1] 王东明[1] 富瑶瑶[1] 胡雪莲[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2011年第4期235-237,共3页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(2007T006;2008T008;2009T007)

摘　　要：以10 kg麦冬为原料,采用甲醇浸提法提取,经过脱脂、脱糖处理后得到18.1 g较纯的麦冬总皂苷,得率为0.181%。通过硅胶柱层析法分离纯化麦冬皂苷D,以氯仿-甲醇为洗脱剂进行洗脱,得到麦冬皂苷D样品2.31 g,其收率为12.8%,经高效液相色谱检测,分离得到的麦冬皂苷D的纯度为91.2%。结果表明,用硅胶柱层析法可快速分离出纯度较高的麦冬皂苷D。18. 1 g ophiopogon total saponins were extracted from 10 kg Ophiopogon japonicus by methanol, the yield of which is 0.18%. Ophiopogon total saponins were separated on silica gel column using chloroform-methanol as eluting agent at different gradient and 2.31 g ophiopognin D was obtained, the yield of which is 12.8%. HPLC analysis was used to detect the purity of ophiopogonin D, the purity was not less than 91.2%. The result proved that silica gel column could quickly isolate high purity ophiopogonin D.

关 键 词：麦冬皂苷D 薄层层析 硅胶柱层析 高效液相色谱 

分 类 号：Q946.83[生物学—植物学] TQ461[化学工程—制药化工]